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anti-ZAP70 antibody :: Mouse ZAP70 Monoclonal Antibody

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Catalog # MBS4380513
Unit / Price
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  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $340 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $340 +1 FREE 8GB USB
Product Name

ZAP70, Monoclonal Antibody

Popular Item
Full Product Name

ZAP70 (Chronic Lymphocytic Leukemia Marker)

Product Synonym Names
Selective T cell defect; SRK; STD; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; TZK; Zeta chain associated protein kinase 70kDa
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P43403
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
ZAP70/2035
Host
Mouse
Species Reactivity
Human. Others not known
Specificity
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Immunogen
Recombinant fragment of human ZAP70 protein (around aa 247-382)
Cellular Localization
Cell Surface and Cytoplasmic
Hu-chromosome Location
2q12
Positive Control
Jurkat cells. Tonsil or lymph node.
Other Notes
Small volumes of anti-ZAP70 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ZAP70 antibody
ELISA (EIA), Immunohistochemistry (IHC) Formalin
Application Notes for anti-ZAP70 antibody
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-ZAP70 antibody
Formalin-fixed, paraffin-embedded human Tonsil stained with ZAP70 Mouse Monoclonal Antibody (ZAP70/2035).
anti-ZAP70 antibody Immunohistochemistry (IHC) (IHC) image
SDS-PAGE Analysis Purified ZAP70 Mouse Monoclonal Antibody (ZAP70/20535).Confirmation of Integrity and Purity of Antibody.
anti-ZAP70 antibody SDS-Page image
Testing Data of anti-ZAP70 antibody
Analysis of Protein Array containing more than 19, 000 full-length human proteins using ZAP70 Mouse Monoclonal Antibody (ZAP70/2035).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
anti-ZAP70 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for ZAP70. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
70kDa
NCBI Official Full Name
tyrosine-protein kinase ZAP-70 isoform 1
NCBI Official Synonym Full Names
zeta chain of T cell receptor associated protein kinase 70
NCBI Official Symbol
ZAP70  [Similar Products]
NCBI Official Synonym Symbols
SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
  [Similar Products]
NCBI Protein Information
tyrosine-protein kinase ZAP-70
UniProt Protein Name
Tyrosine-protein kinase ZAP-70
UniProt Synonym Protein Names
70 kDa zeta-chain associated protein; Syk-related tyrosine kinase
Protein Family
UniProt Gene Name
ZAP70  [Similar Products]
UniProt Synonym Gene Names
NCBI Summary for ZAP70
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ZAP70
Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).
Product References and Citations for anti-ZAP70 antibody
Iwashima M, Irving BA, van Oers NSC, Chan AC, Weiss A. Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases. Science 1994; 263:1136-9.

Precautions
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Disclaimer
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