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anti-CD177 antibody :: Mouse CD177 Monoclonal Antibody

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Catalog # MBS245155
Unit / Price
  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

CD177, Monoclonal Antibody

Full Product Name

Mouse Monoclonal [clone MEM-166] (IgG1) to Human CD177

Product Synonym Names
Anti-CD177 Antibody (clone MEM-166) IHC-plus; CD177; CD177 molecule; CD177 antigen; Cell surface receptor; HNA-2a; HNA2A; NB1; PRV-1; Polycythemia rubra vera 1; PRV1; NB1 glycoprotein; NB1 GP; Human CD177
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
162860
3D Structure
ModBase 3D Structure for Q8N6Q3
Clonality
Monoclonal
Isotype
IgG1
Clone Number
MEM-166
Host
Mouse
Species Reactivity
Human, Monkey
Specificity
Human granulocytes
Purity/Purification
Affinity Purified
Form/Format
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
Concentration
0.5 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Cells.
Immunogen
CD177 antibody was raised against cells.
Preparation and Storage
+4 degree C. Store undiluted.
Other Notes
Small volumes of anti-CD177 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD177 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-CD177 antibody
Flo (1 ug/10E6 cells), IHC-P (10 ug/ml), IP, WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-CD177 antibody
Anti-CD177 antibody IHC of human intravascular neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-CD177 antibody Immunohistochemistry (IHC) (IHC) image
Flow Cytometry (FC/FACS) of anti-CD177 antibody
Human peripheral blood granulocytes stained with purified MEM-166, followed by anti-mouse IgG FITC
anti-CD177 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for CD177. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,683 Da
NCBI Official Full Name
CD177 antigen
NCBI Official Synonym Full Names
CD177 molecule
NCBI Official Symbol
CD177  [Similar Products]
NCBI Official Synonym Symbols
NB1; PRV1; HNA2A; PRV-1; HNA-2a; NB1 GP
  [Similar Products]
NCBI Protein Information
CD177 antigen; NB1 glycoprotein; cell surface receptor; human neutrophil alloantigen 2a; polycythemia rubra vera protein 1
UniProt Protein Name
CD177 antigen
UniProt Synonym Protein Names
Human neutrophil alloantigen 2a; HNA-2a; NB1 glycoprotein; NB1 GP; Polycythemia rubra vera protein 1; PRV-1; CD_antigen: CD177
Protein Family
UniProt Gene Name
CD177  [Similar Products]
UniProt Synonym Gene Names
NB1; PRV1; HNA-2a; NB1 GP; PRV-1  [Similar Products]
UniProt Entry Name
CD177_HUMAN
NCBI Summary for CD177
This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified. [provided by RefSeq, Apr 2014]
UniProt Comments for CD177
CD177: Induced by CSF3 in resting granulocytes. Induced in patients with polycythemia vera (PV) and with essential thrombocythemia (ET). 3 isoforms of the human protein are produced by alternative splicing

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: plasma membrane

Biological Process: blood coagulation; leukocyte migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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