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anti-RPA1 antibody :: Rat RPA, p70 Monoclonal Antibody

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Catalog # MBS604298
Unit / Price
  0.1 mL  /  $540 +1 FREE 8GB USB
anti-RPA1 antibody
Product Name

RPA, p70 (RPA1), Monoclonal Antibody

Also Known As

RPA, p70 (DNA Replication, Repair and Recombination Factor)

Product Synonym Names
Anti -RPA, p70 (DNA Replication, Repair and Recombination Factor)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 17; NC_000017.10 (1733273..1802848). Location: 17p13.3
OMIM
179835
3D Structure
ModBase 3D Structure for P27694
Clonality
Monoclonal
Clone Number
7H59
Host
Rat
Species Reactivity
Human, Monkey
Specificity
Detects endogenous levels of total RPA70 protein. Species Crossreactivity: Human and monkey.
Purity/Purification
Serum
Serum.
Form/Format
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
Immunogen
Recombinant MBP-fusion protein corresponding to the carboxy-terminal sequence of human.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-RPA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RPA1 antibody
RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14kD subunits, collectively known as RPA. RPA is a single stranded DNA binding protein, whose DNA binding activity is believed to reside entirely in the 70kD subunit. The complex is required for almost all aspects of cellular DNA metabolism such as DNA replication (1,2,3), recombination, cell cycle and DNA damage checkpoints, and all major types of DNA repair including nucleotide excision, base excision, mismatch and double-strand break repairs (4,5,6,7). In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 (9-1-1) checkpoint complex (8). RPA is hyperphosphorylated upon DNA damage or replication stress by checkpoint kinases including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) (9,10,11). Hyperphosphorylation may alter RPA-DNA and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA and Pol, and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen (12,10).
Applications Tested/Suitable for anti-RPA1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes for anti-RPA1 antibody
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation.
Dilution: Western blot 1:1000
Immunoprecipitation 1:50
Immunofluorescence (IF-IC) 1:100
NCBI/Uniprot data below describe general gene information for RPA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
68,138 Da[Similar Products]
NCBI Official Full Name
replication protein A 70 kDa DNA-binding subunit
NCBI Official Synonym Full Names
replication protein A1, 70kDa
NCBI Official Symbol
NCBI Official Synonym Symbols
HSSB; RF-A; RP-A; REPA1; RPA70; MST075
  [Similar Products]
NCBI Protein Information
replication protein A 70 kDa DNA-binding subunit; MSTP075; RP-A p70; RF-A protein 1; OTTHUMP00000115691; replication factor A protein 1; single-stranded DNA-binding protein
UniProt Protein Name
Replication protein A 70 kDa DNA-binding subunit
UniProt Synonym Protein Names
Replication factor A protein 1; RF-A protein 1; Single-stranded DNA-binding protein
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
REPA1; RPA70  [Similar Products]
UniProt Entry Name
RFA1_HUMAN
UniProt Comments for RPA1
RPA1: Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Heterotrimer composed of RPA1, RPA2 and RPA3 (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with RIPK1 and XPA. Interacts with RPA4. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interact with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitement to chromatin following DNA damage. Belongs to the replication factor A protein 1 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: male germ cell nucleus; nucleoplasm; lateral element; PML body; DNA replication factor A complex; nucleus

Molecular Function: protein binding; metal ion binding; damaged DNA binding; chromatin binding; single-stranded DNA binding

Biological Process: meiosis; mismatch repair; in utero embryonic development; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; DNA recombination; telomere maintenance via semi-conservative replication; DNA recombinase assembly; homeostasis of number of cells within a tissue; nucleotide-excision repair; base-excision repair; double-strand break repair; transcription-coupled nucleotide-excision repair; positive regulation of cell proliferation; telomere maintenance via recombination; nucleotide-excision repair, DNA damage removal; hemopoiesis; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; DNA-dependent DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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