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anti-RPA1 antibody :: Mouse RPA1 Monoclonal Antibody

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Catalog # MBS9602588
Unit / Price
  0.05 mL  /  $235 +1 FREE 8GB USB
  0.1 mL  /  $295 +1 FREE 8GB USB
  0.2 mL  /  $370 +1 FREE 8GB USB
Western Blot (WB)
Product Name

RPA1, Monoclonal Antibody

Popular Item
Also Known As

RPA1 Antibody

Product Synonym Names
A190; AFDCIN; DNA-directed RNA polymerase I largest subunit; DNA-directed RNA polymerase I subunit A; DNA-directed RNA polymerase I subunit rpa1; Polr1a; Polymerase (RNA) I polypeptide A 194kDa; RNA polymerase I 194 kDa subunit; RNA polymerase I subunit A; RNA polymerase I subunit A1; RPA1; RPA1_HUMAN; RPA194; RPO1 4; RPO14
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1720
3D Structure
ModBase 3D Structure for O95602
Clonality
Monoclonal
Isotype
IgG1
Host
Mouse
Species Reactivity
Human, Monkey
Specificity
RPA1 antibody detects endogenous levels of total RPA1
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
Purified recombinant fragment of human RPA1 expressed in E. Coli
Subcellular Location
Nucleus > Nucleolus.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-RPA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RPA1 antibody
Description: This gene Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair.
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).
Subunit Structure: Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits (PubMed:16809778). Interacts with MYO1C (By similarity). Interacts with ERBB2 (PubMed:21555369). Interacts with DDX11 (PubMed:26089203).
Similarity: Belongs to the RNA polymerase beta' chain family.
Applications Tested/Suitable for anti-RPA1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes for anti-RPA1 antibody
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
ICC: 1:200-1:1000
FC/FACS: 1:200-1:400

Western Blot (WB) of anti-RPA1 antibody
Figure 1: Western blot analysis using RPA1 mAb against human RPA1 (AA: 308-513) recombinant protein. (Expected MW is 48.3 kDa)
anti-RPA1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for RPA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 68.1 kDa
Predicted: 195 kDa
NCBI Official Full Name
DNA-directed RNA polymerase I subunit RPA1
NCBI Official Synonym Full Names
RNA polymerase I subunit A
NCBI Official Symbol
POLR1A  [Similar Products]
NCBI Official Synonym Symbols
A190; RPA1; RPO14; AFDCIN; RPA194; RPO1-4
  [Similar Products]
NCBI Protein Information
DNA-directed RNA polymerase I subunit RPA1
UniProt Protein Name
DNA-directed RNA polymerase I subunit RPA1
UniProt Synonym Protein Names
A190; DNA-directed RNA polymerase I largest subunit; DNA-directed RNA polymerase I subunit A; RNA polymerase I 194 kDa subunit; RPA194
Protein Family
UniProt Gene Name
POLR1A  [Similar Products]
UniProt Synonym Gene Names
RNA polymerase I subunit A1; RPA194  [Similar Products]
NCBI Summary for RPA1
The protein encoded by this gene is the largest subunit of the RNA polymerase I complex. The encoded protein represents the catalytic subunit of the complex, which transcribes DNA into ribosomal RNA precursors. Defects in this gene are a cause of the Cincinnati type of acrofacial dysostosis. [provided by RefSeq, May 2016]
UniProt Comments for RPA1
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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