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anti-ILK antibody :: Mouse ILK Monoclonal Antibody

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Catalog # MBS9602312
Unit / Price
  0.05 mL  /  $235 +1 FREE 8GB USB
  0.1 mL  /  $295 +1 FREE 8GB USB
  0.2 mL  /  $370 +1 FREE 8GB USB
Western Blot (WB)
Product Name

ILK, Monoclonal Antibody

Popular Item
Also Known As

ILK Antibody

Product Synonym Names
59 kDa serine/threonine protein kinase; 59 kDa serine/threonine-protein kinase; DKFZp686F1765; Epididymis secretory protein Li 28; HEL S 28; ILK 1; ILK 2; ILK; ILK-1; ILK-2; ILK_HUMAN; ILK1; ILK2; Integrin linked kinase 2; Integrin linked Kinase; Integrin linked protein kinase; Integrin-linked protein kinase; p59; p59ILK
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
452
3D Structure
ModBase 3D Structure for Q13418
Clonality
Monoclonal
Isotype
IgG1
Host
Mouse
Species Reactivity
Human, Mouse, Monkey
Specificity
ILK antibody detects endogenous levels of total ILK
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
Purified recombinant fragment of human ILK expressed in E. Coli
Subcellular Location
Cell Junction > Focal Adhesion. Cell Membrane.
Tissue Specificity
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-ILK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ILK antibody
Description: Influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin.
Function: Receptor-proximal protein kinase regulating integrin-mediated signal transduction (PubMed:8538749, PubMed:9736715). May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin-and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (PubMed:8538749, PubMed:9736715).
Subunit Structure: Interacts with FERMT2 (By similarity). Interacts with the cytoplasmic domain of ITGB1. Could also interact with integrin ITGB2, ITGB3 and/or ITGB5. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with PARVA and PARVB; these compete for the same binding site. Interacts probably also with TGFB1I1. Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1. Interacts with LIMD2; leading to activate the protein kinase activity (PubMed:24590809).
Post-translational Modifications: Autophosphorylated on serine residues.
Similarity: A PH-like domain is involved in phosphatidylinositol phosphate binding. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Applications Tested/Suitable for anti-ILK antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes for anti-ILK antibody
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
FC/FACS: 1:200-1:400

Western Blot (WB) of anti-ILK antibody
Figure 1: Western blot analysis using ILK mAb against human ILK recombinant protein. (Expected MW is 42.7 kDa)
anti-ILK antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ILK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 51.4 kDa
Predicted: 52 kDa
NCBI Official Full Name
integrin-linked protein kinase isoform 1
NCBI Official Synonym Full Names
integrin linked kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
P59; ILK-1; ILK-2; p59ILK; HEL-S-28
  [Similar Products]
NCBI Protein Information
integrin-linked protein kinase
UniProt Protein Name
Integrin-linked protein kinase
UniProt Synonym Protein Names
59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK
UniProt Gene Name
UniProt Synonym Gene Names
ILK1; ILK2  [Similar Products]
NCBI Summary for ILK
This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
UniProt Comments for ILK
Receptor-proximal protein kinase regulating integrin-mediated signal transduction (PubMed:8538749, PubMed:9736715). May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (PubMed:8538749, PubMed:9736715).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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