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anti-BAX antibody :: Mouse BAX Monoclonal Antibody

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Catalog # MBS245269
Unit / Price
  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

BAX, Monoclonal Antibody

Full Product Name

Mouse Monoclonal [clone 6A7] (IgG1) to Human BAX

Product Synonym Names
Anti-BAX Antibody (clone 6A7) IHC-plus; BAX; BCL2-associated X protein; Bcl2-L-4; Apoptosis regulator BAX; Bcl-2-like protein 4; BCL2L4; Human BAX
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
600040
3D Structure
ModBase 3D Structure for Q07812
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6A7
Host
Mouse
Species Reactivity
Human, Monkey, Mouse, Rat
Specificity
Amino acid:12-24 common to human, mouse, and rat Bax protein.
Purity/Purification
Affinity Purified
Form/Format
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide at 0.5 mg/ml.
Concentration
0.5 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Synthetic peptide from human BAX.
Immunogen Type
Synthetic peptide
Immunogen
BAX antibody was raised against synthetic peptide from human BAX.
Preparation and Storage
+4 degree C. Store undiluted.
Other Notes
Small volumes of anti-BAX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BAX antibody
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death.
Product Categories/Family for anti-BAX antibody
Applications Tested/Suitable for anti-BAX antibody
Immunohistochemistry (IHC - Paraffin), Immunoprecipitation (IP)
Application Notes for anti-BAX antibody
IHC-P (10 - 12 ug/ml), IP, WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-BAX antibody
Anti-BAX antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-BAX antibody Immunohistochemistry (IHC) (IHC) image
Immunoprecipitation (IP) of anti-BAX antibody
Extracts from Hela cell lysate was immunoprecipitated with clone 6A7 (lane 1), 4 ug purified monoclonal antibody without cell lysate (clone 6A7, lane 2), or Hela cell lysate without immunoprecipitation (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Bax antibody, poly6251.
anti-BAX antibody Immunoprecipitation (IP) (IP) image
NCBI/Uniprot data below describe general gene information for BAX. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
19,718 Da
NCBI Official Full Name
apoptosis regulator BAX isoform beta
NCBI Official Synonym Full Names
BCL2-associated X protein
NCBI Official Symbol
NCBI Official Synonym Symbols
BCL2L4
  [Similar Products]
NCBI Protein Information
apoptosis regulator BAX; bcl2-L-4; Baxdelta2G9; Baxdelta2omega; Baxdelta2G9omega; bcl-2-like protein 4; BCL2-associated X protein omega
UniProt Protein Name
Apoptosis regulator BAX
UniProt Synonym Protein Names
Bcl-2-like protein 4; Bcl2-L-4
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
BCL2L4; Bcl2-L-4  [Similar Products]
UniProt Entry Name
BAX_HUMAN
NCBI Summary for BAX
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for BAX
BAX: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with human cytomegalovirus/HHV-5 protein vMIA/UL37. Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Belongs to the Bcl-2 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Apoptosis; Tumor suppressor; Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial permeability transition pore complex; pore complex; endoplasmic reticulum membrane; mitochondrial outer membrane; membrane; mitochondrion; endoplasmic reticulum; nuclear envelope; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; channel activity; BH3 domain binding; lipid binding

Biological Process: hypothalamus development; viral reproduction; regulation of cell cycle; positive regulation of apoptosis; response to toxin; myeloid cell homeostasis; germ cell programmed cell death; B cell apoptosis; germ cell development; regulation of mammary gland epithelial cell proliferation; spermatid differentiation; regulation of mitochondrial membrane potential; development of secondary sexual characteristics; protein insertion into mitochondrial membrane during induction of apoptosis; establishment and/or maintenance of transmembrane electrochemical gradient; negative regulation of neuron apoptosis; negative regulation of protein binding; kidney development; release of cytochrome c from mitochondria; positive regulation of B cell apoptosis; regulation of protein homodimerization activity; vagina development; protein oligomerization; fertilization; unfolded protein response, activation of signaling protein activity; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; retina development in camera-type eye; negative regulation of fibroblast proliferation; reduction of endoplasmic reticulum calcium ion concentration; glycosphingolipid metabolic process; mitochondrial fragmentation during apoptosis; cerebral cortex development; regulation of nitrogen utilization; post-embryonic camera-type eye morphogenesis; positive regulation of pigmentation; regulation of protein heterodimerization activity; T cell homeostatic proliferation; apoptosis; neuron migration; negative regulation of peptidyl-serine phosphorylation; positive regulation of apoptosis involved in mammary gland involution; release of matrix enzymes from mitochondria; response to salt stress; positive regulation of protein oligomerization; apoptotic mitochondrial changes; B cell homeostatic proliferation; ovarian follicle development; B cell homeostasis; positive regulation of neuron apoptosis; response to gamma radiation; response to axon injury; B cell negative selection; protein homooligomerization; caspase activation; transformed cell apoptosis; mitochondrial fusion; Sertoli cell proliferation; odontogenesis of dentine-containing teeth; endoplasmic reticulum calcium ion homeostasis; neuron apoptosis; homeostasis of number of cells within a tissue; retinal cell programmed cell death; blood vessel remodeling; caspase activation via cytochrome c; positive regulation of release of sequestered calcium ion into cytosol
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