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anti-CD68 antibody :: Mouse CD68 Antibody

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Catalog # MBS438131
Unit / Price
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  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $200 +1 FREE 8GB USB
  7 mL (Prediluted)  /  $285 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $315 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $325 +1 FREE 8GB USB
Product Name

CD68, Antibody

Popular Item
Full Product Name

CD68 (Macrophage Marker) Mouse Monoclonal Antibody

Product Synonym Names
GP110, LAMP4, Microsialin, Macrosialin, SCARD1, Scavenger Receptor Class D Member-1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
153634
3D Structure
ModBase 3D Structure for P34810
Isotype
IgG1, kappa
Host
Mouse
Species Reactivity
Human, Monkey, Mouse, and Rat. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Human Unigene
647419
Hu-Chromosome Location
17p13.1
Immunogen
Recombinant human CD68 protein
Cellular Localization
Cell Surface and Cytoplasmic
Positive Control
U87MG cells,Tonsil, Lymph Node or Spleen
Preparation and Storage
Store at 2-8°C.
Other Notes
Small volumes of anti-CD68 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD68 antibody
This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
Applications Tested/Suitable for anti-CD68 antibody
Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC), Immunohistology (Formalin-fixed)
Application Notes for anti-CD68 antibody
Western blotting (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-CD68 antibody
Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68 Monoclonal Antibody (C68/684).
anti-CD68 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CD68 antibody
Formalin-fixed, paraffin-embedded human Tonsil stained with CD68 Monoclonal Antibody (C68/684).
anti-CD68 antibody Immunohistochemistry (IHC) (IHC) image
SDS-PAGE of anti-CD68 antibody
SDS-PAGE Analysis of Purified CD68 Monoclonal Antibody (C68/684).
anti-CD68 antibody SDS-PAGE image
Western Blot (WB) of anti-CD68 antibody
Western Blot Analysis of human Spleen tissue lysate using CD68 Monoclonal Antibody (C68/684).
anti-CD68 antibody Western Blot (WB) (WB) image
Flow Cytometry (FC) of anti-CD68 antibody
Flow Cytometric Analysis of U87MG cells using CD68 Monoclonal Antibody (C68/684); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
anti-CD68 antibody Flow Cytometry (FC) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for CD68. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
110kDa
NCBI Official Full Name
macrosialin isoform B
NCBI Official Synonym Full Names
CD68 molecule
NCBI Official Symbol
NCBI Official Synonym Symbols
GP110; LAMP4; SCARD1
  [Similar Products]
NCBI Protein Information
macrosialin
UniProt Protein Name
Macrosialin
UniProt Synonym Protein Names
Gp110; CD_antigen: CD68
Protein Family
UniProt Gene Name
UniProt Entry Name
CD68_HUMAN
NCBI Summary for CD68
This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Comments for CD68
CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: membrane
Product References and Citations for anti-CD68 antibody
Pulford KA et. al. Journal of Clinical Pathology, 1989, 42(4):414-21. Warnke RA et. al. Am J of Pathol, 1989, 135:1089-95.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-CD68 antibody
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