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anti-CDK5 antibody :: Mouse CDK5 Monoclonal Antibody

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Catalog # MBS475069
Unit / Price
  0.1 mL  /  $335 +1 FREE 8GB USB
Product Name

CDK5, Monoclonal Antibody

Popular Item
Full Product Name

Anti-CDK5 (N-term) Mouse mAb

Product Synonym Names
Cdk 5; Cdk5; CDK5_HUMAN; Cell division protein kinase 5; Crk6; Cyclin dependent kinase 5; Cyclin-dependent kinase 5; Protein kinase CDK5 splicing; PSSALRE; Serine threonine protein kinase PSSALRE; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
X66364 mRNA
3D Structure
ModBase 3D Structure for Q00535
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2E8-F9-B7-C11
Host
Mouse
Species Reactivity
Human, Mouse, Rat, Monkey
Form/Format
Ascites
Concentration
1mg/ml (lot specific)
Immunogen
Purified recombinant human CDK5 (N-terminus) protein fragments expressed in E Coli.
Sensitivity
This antibody detects endogenous levels of CDK5 (N-terminus) and does not cross-react with related proteins.
Sequence Similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain
UniPort Summary Function
Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
Subunit Structure
Heterodimer composed of a catalytic subunit CDK5 and a regulatory subunit CDK5R1 (p25) and macromolecular complex composed of at least CDK5, CDK5R1 (p35) and CDK5RAP1 or CDK5RAP2 or CDK5RAP3. Only the heterodimer shows kinase activity. Under neurotoxic stress and neuronal injury conditions, p35 is cleaved by calpain to generate p25 that hyperactivates CDK5, that becomes functionally disabled and often toxic. Found in a trimolecular complex with CABLES1 and ABL1. Interacts with CABLES1 and CABLES2. Interacts with AATK and GSTP1. Binds to HDAC1 when in complex with p25. Interaction with myristoylation p35 promotes CDK5 association with membranes. Both isoforms 1 and 2 interacts with beta-catenin/CTNNB1. Interacts with delta-catenin/CTNND2 and APEX1. Interacts with P53/TP53 in neurons. Interacts with EPHA4; may mediate the activation of NGEF by EPHA4
Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein. Perikaryon. Cell projection
Antibody diluents
5% w/v nonfat dry milk, 1x PBS, 0.1%Tween-20
Preparation and Storage
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles
Other Notes
Small volumes of anti-CDK5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CDK5 antibody
Cdks (cyclin-dependent kinases) are heteromeric serine/threonine kinases that control progression through the cell cycle in concert with their regulatory subunits, the cyclins. Although there are 12 different cdk genes, only 5 have been shown to directly drive the cell cycle (Cdk1, -2, -3, -4, and -6). Following extracellular mitogenic stimuli, cyclin D gene expression is upregulated. Cdk4 forms a complex with cyclin D and phosphorylates Rb protein, leading to liberation of the transcription factor E2F. E2F induces transcription of genes including cyclins A and E, DNA polymerase and thymidine kinase. Cdk4-cyclin E complexes form and initiate G1/S transition. Subsequently, Cdk1-cyclin B complexes form and induce G2/M phase transition. Cdk1-cyclin B activation induces the breakdown of the nuclear envelope and the initiation of mitosis. Cdks are constitutively expressed and are regulated by several kinases and phosphastases, including Wee1, CDK-activating kinase and Cdc25 phosphatase. In addition, cyclin expression is induced by molecular signals at specific points of the cell cycle, leading to activation of Cdks. Tight control of Cdks is essential as misregulation can induce unscheduled proliferation, and genomic and chromosomal instability. Cdk4 has been shown to be mutated in some types of cancer, whilst a chromosomal rearrangement can lead to Cdk6 overexpression in lymphoma, leukemia and melanoma. Cdks are currently under investigation as potential targets for antineoplastic therapy, but as Cdks are essential for driving each cell cycle phase, therapeutic strategies that block Cdk activity are unlikely to selectively target tumor cells.
Applications Tested/Suitable for anti-CDK5 antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-CDK5 antibody
WB: 1:500
ICC: 1:150

Western Blot (WB) of anti-CDK5 antibody
Western blot detection of CDK5 (N-terminus) in 3T3, Hela, PC-12, COS7, Jurkat and K562 lysates using Cdk5 mouse mAb (1:500 diluted).Predicted band size: 36KDa.Observed band size: 36KDa.Exposure time:5min.
anti-CDK5 antibody Western Blot (WB) (WB) image
Immunocytochemistry (ICC) of anti-CDK5 antibody
Immunocytochemistry of HeLa cells using anti-CDK5 (N-terminus) mouse mAb diluted 1:150
anti-CDK5 antibody Immunocytochemistry (ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for CDK5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
36
NCBI Official Full Name
cyclin-dependent-like kinase 5 isoform 2
NCBI Official Synonym Full Names
cyclin dependent kinase 5
NCBI Official Symbol
NCBI Official Synonym Symbols
LIS7; PSSALRE
  [Similar Products]
NCBI Protein Information
cyclin-dependent-like kinase 5
UniProt Protein Name
Cyclin-dependent-like kinase 5
UniProt Synonym Protein Names
Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CDKN5; TPKII catalytic subunit  [Similar Products]
NCBI Summary for CDK5
This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
UniProt Comments for CDK5
CDK5: a protein kinase of the CDK family. Unlike other members of this family, it is not activated by cyclins but by p35 (CDK5R1) and p39. An important regulator of neuronal positioning during brain development. May also play a role in synaptogenesis and neurotransmission. Substrates include TAU, MAP2, NF-H and -M, Nudel, PDE6, beta-catenin, amphyphysin, dynamin I, synapsin 1, Munc-18, and NMDA receptor 2A. Plays a role in myogenesis, haematopoietic cell differentiation, spermatogenesis, insulin secretion, and lens differentiation. Implicated in the pathology of neurofibrillary tangles and formation of senile plaques, hallmarks of Alzheimer?s disease. Induces tau phosphorylation and aggregation and neurofibrillary tangle deposition and neurodegeneration in in vitro and in vivo animal models. Brain samples from Alzeimer?s pateints show elevated CDK5 activity.

Protein type: CDK family; CDK/CDK5 subfamily; CDK5 subfamily; CMGC group; Cell cycle regulation; EC 2.7.11.1; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: axon; cell junction; cell soma; cytoplasm; cytosol; dendrite; growth cone; membrane; neuromuscular junction; nucleoplasm; nucleus; plasma membrane; postsynaptic density

Molecular Function: acetylcholine receptor activator activity; cyclin-dependent protein kinase activity; ErbB-2 class receptor binding; ErbB-3 class receptor binding; kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity; tau-protein kinase activity

Biological Process: axon extension; cell proliferation; negative regulation of proteolysis; negative regulation of transcription, DNA-dependent; neurite development; neuron apoptosis; neuron differentiation; neuron migration; oligodendrocyte differentiation; peptidyl-serine phosphorylation; phosphorylation; positive regulation of neuron apoptosis; regulation of apoptosis; regulation of macroautophagy; regulation of synaptic plasticity; synaptic transmission; synaptic vesicle endocytosis; synaptic vesicle exocytosis; synaptogenesis

Disease: Lissencephaly 7 With Cerebellar Hypoplasia
Precautions
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