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anti-SIRT6 antibody :: Mouse SIRT6 Monoclonal Antibody

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Catalog # MBS475406
Unit / Price
  0.1 mL  /  $335 +1 FREE 8GB USB
Product Name

SIRT6, Monoclonal Antibody

Popular Item
Full Product Name

Anti-SIRT6 Mouse mAb

Product Synonym Names
2810449N18Rik; AI043036; Mono ADP ribosyltransferase sirtuin 6; NAD-dependent protein deacetylase sirtuin-6; Regulatory protein SIR2 homolog 6; Regulatory protein SIR2 homolog; SIR2 like 6; SIR2 like protein 6; Sir2 related protein type 6; SIR2-like protein 6; SIR2
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
AF233396 mRNA
3D Structure
ModBase 3D Structure for Q8N6T7
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6C9-D10-D3
Host
Mouse
Species Reactivity
Human, Mouse, Rat, Monkey
Purity/Purification
Affinity Purified
Form/Format
PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Immunogen
Purified recombinant human SIRT6 protein expressed in E Coli.
Sensitivity
This antibody detects endogenous levels of SIRT6 and does not cross-react with related proteins.
Sequence Similarities
Belongs to the sirtuin family. Class IV subfamily.Contains 1 deacetylase sirtuin-type domain.
Subcellular Location
Nucleus
Antibody Diluents
5% w/v nonfat dry milk, 1 x PBS, 0.1%Tween-20
Preparation and Storage
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles
Other Notes
Small volumes of anti-SIRT6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SIRT6 antibody
Entrez Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been det
Applications Tested/Suitable for anti-SIRT6 antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-SIRT6 antibody
WB: 1:500
ICC: 1:100

Western Blot (WB) of anti-SIRT6 antibody
Western blot analysis of extracts from Jurkat, MCF7, HCT116, K562, COS7, C2C12, 3T3 and Hela cell lysates using SIRT6 mouse mAb (1:500 diluted).Predicted band size:42, 36KDa.Observed band size:42, 36KDa.
anti-SIRT6 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-SIRT6 antibody
Immunofluorescent analysis of Hela cells fixed fixed by 4% paraformaldehyde and using SIRT6 mouse mAb (dilution 1:100).
anti-SIRT6 antibody Immunofluorescence (IF) image
Immunoprecipitation (IP) of anti-SIRT6 antibody
Immunoprecipitation analysis of Hela cell lysates using SIRT6 mouse mAb.
anti-SIRT6 antibody Immunoprecipitation (IP) (IP) image
NCBI/Uniprot data below describe general gene information for SIRT6. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42
NCBI Official Full Name
NAD-dependent protein deacetylase sirtuin-6 isoform 2
NCBI Official Synonym Full Names
sirtuin 6
NCBI Official Symbol
SIRT6  [Similar Products]
NCBI Official Synonym Symbols
SIR2L6
  [Similar Products]
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-6
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-6
UniProt Synonym Protein Names
Regulatory protein SIR2 homolog 6; SIR2-like protein 6
UniProt Gene Name
SIRT6  [Similar Products]
UniProt Synonym Gene Names
SIR2L6  [Similar Products]
NCBI Summary for SIRT6
This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
UniProt Comments for SIRT6
SIRT6: NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. Interacts with RELA. Interacts with RBBP8; the interaction deacetylates RBBP8. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Deacetylase; EC 2.4.2.31; EC 3.5.1.-; Transferase

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nuclear telomeric heterochromatin; nucleolus; nucleoplasm; nucleus

Molecular Function: NAD(P)+-protein-arginine ADP-ribosyltransferase activity; NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; zinc ion binding

Biological Process: positive regulation of chromatin silencing at telomere; positive regulation of telomere maintenance; protein amino acid ADP-ribosylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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