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anti-CHRM2 antibody :: Rat Muscarinic Acetylcholine Receptor M2, aa225-359 Monoclonal Antibody

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Catalog # MBS602250
Unit / Price
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  0.1 mg  /  $665 +1 FREE 8GB USB
anti-CHRM2 antibody
Product Name

Muscarinic Acetylcholine Receptor M2, aa225-359 (CHRM2), Monoclonal Antibody

Full Product Name

Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007)

Product Synonym Names
Anti -Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 7; NC_000007.13 (136553399..136701771). Location: 7q31-q35
OMIM
103780
3D Structure
ModBase 3D Structure for P08172
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
2Q147
Host
Rat
Species Reactivity
Human, Monkey, Rat
Specificity
M2 muscarinic acetylcholine receptor. No reactivity with the other subtypes. Species crossreactivity: Human, monkey and rat. Expected to react with most mammalian species.
Purity/Purification
Purified
Purified immunoglobulin
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Immunogen
i3 loop of m2 receptor fusion protein (225-359), fused to Glutathione S-transferase.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

IMMUNOHISTOCHEMISTRY PROTOCOL
Use on 30 um, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation.
1. 3 x 10 minute washes in TBS (with or without 0.25% Triton).
2. Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3. Incubate M9520-13B diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at RT followed by 16 hours at 4 degree C.
4. 3 x 10 minute washes in TBS.
5. Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).
6. 3 x 10 minute washes in TBS.
7. ABC Elite (1:200) in TBS.
8. 2 x 10 minute washes in TBS.
9. 1 x 10 minute wash in phosphate buffer (no saline).
10. DAB reaction with 0.06% NiCl added for intensification.
11. 2 x 10 minute washes in PBS.
12. 1 x 10 minute wash in phosphate buffer (no saline).
Other Notes
Small volumes of anti-CHRM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CHRM2 antibody
Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for anti-CHRM2 antibody
Suitable for use in Immunohistochemistry and Immunocytochemistry. Does not work on paraffin embedded tissues, and Immunoblotting.
Dilution: Immunohistochemistry (4% paraformaldehyde fixed tissue): 1-5ug/ml
NCBI/Uniprot data below describe general gene information for CHRM2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
51,715 Da[Similar Products]
NCBI Official Full Name
muscarinic acetylcholine receptor M2
NCBI Official Synonym Full Names
cholinergic receptor, muscarinic 2
NCBI Official Symbol
CHRM2  [Similar Products]
NCBI Official Synonym Symbols
HM2; FLJ43243; MGC120006; MGC120007
  [Similar Products]
NCBI Protein Information
muscarinic acetylcholine receptor M2; 7TM receptor; OTTHUMP00000208393; OTTHUMP00000208394; OTTHUMP00000208395; OTTHUMP00000208396; muscarinic M2 receptor; cholinergic receptor, muscarinic 2, isoform a
UniProt Protein Name
Muscarinic acetylcholine receptor M2
UniProt Gene Name
CHRM2  [Similar Products]
UniProt Entry Name
ACM2_HUMAN
NCBI Summary for CHRM2
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq]
UniProt Comments for CHRM2
mAChR M2: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD). MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 7q31-q35

Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction

Molecular Function: G-protein coupled acetylcholine receptor activity

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; nervous system development; G-protein signaling, coupled to cyclic nucleotide second messenger; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); acetylcholine receptor signaling, muscarinic pathway; response to virus; muscarinic acetylcholine receptor, phospholipase C activating pathway; regulation of heart contraction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withanti-CHRM2 antibody
 Reference Product  PubMed Publications
 GRK5 antibody  >1 publications with CHRM2 and GRK5
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