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anti-AAT antibody :: Mouse anti-Human, Mouse AAT Monoclonal Antibody

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Catalog # MBS9602377
Unit / Price
  0.05 mL  /  $235 +1 FREE 8GB USB
  0.1 mL  /  $295 +1 FREE 8GB USB
  0.2 mL  /  $370 +1 FREE 8GB USB
Western Blot (WB)
Product Name

AAT, Monoclonal Antibody

Popular Item
Full Product Name

AAT Antibody

Product Synonym Names
A1A; A1AT; A1AT_HUMAN; AAT; Alpha 1 antiproteinase; Alpha 1 antitrypsin; Alpha 1 antitrypsin null; Alpha 1 protease inhibitor; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; alpha1 proteinase inhibitor; Alpha1AT; Dom1; PI; PI1; PRO2275; Serine (or cysteine) proteinase inhibitor clade A member 1; Serine protease inhibitor 1-1; Serine protease inhibitor A1a; Serpin A1; Serpin A1a; Serpin peptidase inhibitor clade A member 1; Serpina1; Short peptide from AAT; SPAAT; Spi1-1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P01009
Clonality
Monoclonal
Isotype
IgG1
Host
Mouse
Species Reactivity
Human, Mouse
Specificity
AAT antibody detects endogenous levels of total AAT
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
Purified recombinant fragment of human AAT expressed in E. Coli
Subcellular Location
Secreted. Endoplasmic Reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and secreted, extracellular space, extracellular matrix.
Tissue Specificity
Ubiquitous. Expressed in leukocytes and plasma.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-AAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AAT antibody
Description: AAT, also known as SERPINA1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1. It is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Severe Serpin A1 deficiency leads to several clinical complications such as pulmonary emphysema, juvenile hepatisis, cirrhosis and hepatocellular carcinoma.
Function: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Subunit Structure: The variants S and Z interact with CANX AND PDIA3.
Post-translational Modifications: N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
Similarity: The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. Belongs to the serpin family.
Applications Tested/Suitable for anti-AAT antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-AAT antibody
ELISA: 1:10000
WB: 1:500-1:2000

Western Blot (WB) of anti-AAT antibody
Figure 1: Western blot analysis using AAT mouse mAb against human plasma (1) and NIH/3T3 cell lysate (2).
anti-AAT antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for AAT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 47 kDa
Predicted: 47 kDa
NCBI Official Full Name
alpha-1-antitrypsin
NCBI Official Synonym Full Names
serpin family A member 1
NCBI Official Symbol
SERPINA1  [Similar Products]
NCBI Official Synonym Symbols
PI; A1A; AAT; PI1; A1AT; nNIF; PRO2275; alpha1AT
  [Similar Products]
NCBI Protein Information
alpha-1-antitrypsin
UniProt Protein Name
Alpha-1-antitrypsin
UniProt Synonym Protein Names
Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1
UniProt Gene Name
SERPINA1  [Similar Products]
UniProt Synonym Gene Names
AAT; PI; SPAAT  [Similar Products]
NCBI Summary for AAT
The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for AAT
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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