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anti-AK3 antibody :: Mouse anti-Human, Mouse AK3 Monoclonal Antibody

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Catalog # MBS200020
Unit / Price
  0.05 mL  /  $260 +1 FREE 8GB USB
  0.1 mL  /  $350 +1 FREE 8GB USB
Product Name

AK3, Monoclonal Antibody

Also Known As

AK3 antibody

Product Synonym Names
adenylate kinase; Ak6; FIX; Ak3L1; AkL3L; AkL3L1; adenylate kinase 3; adenylate kinase 6; adenylate kinase 3 like 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
227
OMIM
AB021870 mRNA
3D Structure
ModBase 3D Structure for Q9UIJ7
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
SJB3-36
Host
Mouse
Species Reactivity
Human, Mouse
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human Ak3 (adenylaste kinase isozyme 3) purified from E. coli
Clone Note
Anti-human Ak3 mAb, clone SJB3-36, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Ak3 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-AK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AK3 antibody
Adenylate kinase (Ak; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2.7.4.3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (Ak1, Ak2 and Ak3) are characterized in vertebrates. Ak1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while Ak2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. Ak3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell.
Product Categories/Family for anti-AK3 antibody
Applications Tested/Suitable for anti-AK3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-AK3 antibody
WB: 1:500 ~ 1:2,000.
Recommended starting dilution is 1:1,000.

Western Blot (WB) of anti-AK3 antibody
The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK3 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human AK1 Lane 2. : Recombinant Human AK2 Lane 3. : Recombinant Human AK3 The lysate (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human AK3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hep3B cell lysate Lane 2. : 293T cell lysate Lane 3. : Mouse kidney tissue lysate The lysate (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human AK3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Mouse liver tissue lysate
anti-AK3 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-AK3 antibody
Human liver tissue was incubated with anti-human Ak3 (1:100) for 2 hours at room temperature. Slide was then washed in PBS, and was incubated in avidin biosystem anti-rabbit labeled polymer for 30 min at RT. Enzyme detection was performed with DAB chromo-gen.
anti-AK3 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-AK3 antibody
ICC/IF analysis of AK3 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
anti-AK3 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for AK3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,014 Da
NCBI Official Full Name
GTP:AMP phosphotransferase AK3, mitochondrial isoform a
NCBI Official Synonym Full Names
adenylate kinase 3
NCBI Official Symbol
NCBI Official Synonym Symbols
AK6; FIX; AK3L1; AKL3L; AKL3L1
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NCBI Protein Information
GTP:AMP phosphotransferase AK3, mitochondrial
UniProt Protein Name
GTP:AMP phosphotransferase AK3, mitochondrial
UniProt Synonym Protein Names
Adenylate kinase 3
UniProt Gene Name
UniProt Synonym Gene Names
NCBI Summary for AK3
The protein encoded by this gene is a GTP:ATP phosphotransferase that is found in the mitochondrial matrix. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
UniProt Comments for AK3
Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.
Product References and Citations for anti-AK3 antibody
Noda, L.H. et al. (1973) The Enzymes. Vol. 8, 279-305; Khoo, J.C. et al. (1972) Biochim. Biophys. Acta 268, 98-101; Tomasselli, A.G. et al. (1979) Eur.J.Biochemolecules 93, 257-262; Lee, Y. et al. (1998) J. Biochem. 123, 47-54.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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