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anti-EBI3 antibody :: Rat anti-Human, Mouse EBI3 Monoclonal Antibody

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Catalog # MBS245820
Unit / Price
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  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

EBI3, Monoclonal Antibody

Full Product Name

Rat Monoclonal [clone DNT27] (IgG2a,k) to Mouse EBI3

Product Synonym Names
Anti-EBI3 Antibody (clone DNT27) IHC-plus; EBI3; Epstein-Barr virus induced 3; IL-27 subunit beta; IL27 subunit; IL27B; Interleukin-27 subunit beta; IL-27B; Cytokine receptor; EBV-induced gene 3 protein; IL35 subunit; Mouse EBI3
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
605816
3D Structure
ModBase 3D Structure for Q14213
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
DNT27
Host
Rat
Species Reactivity
Human, Mouse
Purity/Purification
Affinity Purified
Form/Format
Aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Concentration
0.5 mg/ml (lot specific)
Target Species
Mouse
Immunogen Description
Mouse EBI3
Preparation and Storage
Store at 4 degree C, avoid repeated freeze thaw cycles.
Other Notes
Small volumes of anti-EBI3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-EBI3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-EBI3 antibody
IHC-P (10 ug/ml), WB
Usage: This DNT27 antibody has been tested by western blot of cell lysates prepared from mouse EBI3-transfected 293T cells and bone marrow-derived dendritic cells. This can be used at less than or equal to 1-5 ug/ml. It is recommended that the antibody be c...

Immunohistochemistry (IHC) of anti-EBI3 antibody
Anti-EBI3 antibody IHC of human lymphoid-dendritic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-EBI3 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-EBI3 antibody
Recombinant mouse EBI3/p28 (covalently linked IL-27) protein (lane 1) and mouse bone marrow-derived dendritic cells stimulated with LPS (lane 2) or left untreated (lane3) were analyzed by SDS-PAGE followed by immunoblotting with 5 ug/ml of anti-mouse EBI3 (DNT27). Bands were visualized with horseradish peroxidase (HRP)-conjugated goat anti-rat IgG.
anti-EBI3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for EBI3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,396 Da
NCBI Official Full Name
interleukin-27 subunit beta
NCBI Official Synonym Full Names
Epstein-Barr virus induced 3
NCBI Official Symbol
NCBI Official Synonym Symbols
IL27B; IL-27B
  [Similar Products]
NCBI Protein Information
interleukin-27 subunit beta; IL27 subunit; IL35 subunit; cytokine receptor; IL-27 subunit beta; EBV-induced gene 3 protein; Epstein-Barr virus induced gene 3; epstein-Barr virus-induced gene 3 protein
UniProt Protein Name
Interleukin-27 subunit beta
UniProt Synonym Protein Names
Epstein-Barr virus-induced gene 3 protein; EBV-induced gene 3 protein
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
IL27B; IL-27 subunit beta; IL-27B; EBV-induced gene 3 protein  [Similar Products]
UniProt Entry Name
IL27B_HUMAN
NCBI Summary for EBI3
This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells. [provided by RefSeq, Sep 2008]
UniProt Comments for EBI3
IL27-beta: Cytokine with pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon- gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines. Belongs to the type I cytokine receptor family. Type 3 subfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; interleukin-27 receptor binding; cytokine activity

Biological Process: T-helper 1 type immune response; cytokine and chemokine mediated signaling pathway; positive regulation of alpha-beta T cell proliferation; humoral immune response; positive regulation of interferon-gamma biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-EBI3 antibody
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