• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-EphA2 antibody :: Mouse anti-Human, Mouse EphA2 Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS200110
Unit / Price
Scan QR to view Datasheet
  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Product Name

EphA2, Monoclonal Antibody

Full Product Name

EphA2 antibody

Product Synonym Names
Ephrin receptor EphA2; epoxide hydrolase 1 microsomal (xenobiotic);
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
M59371 mRNA
3D Structure
ModBase 3D Structure for P29317
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
3D7
Host
Mouse
Species Reactivity
Human, Mouse
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human EphA2 (559-976aa) purified from E. coli
Clone Note
Anti-human EphA2 mAb, clone 3D7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human EphA2 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-EphA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-EphA2 antibody
The Eph family of receptors is the largest known subfamily of receptor tyrosine kinases (RTk). Eph RTks and their ligands, the ephrins, are frequently overexpressed in a variety of cancers and tumor cell lines. One Eph family member, EphA2, is also overexpressed in a wide variety of cancers, including breast, small-cell lung and gastrointestinal cancers, melanomas, and neuroblastomas. The ephrin-Eph interactions are important in development, especially in cell-cell interactions involved in nervous system patterning and possibly in cancer.
Product Categories/Family for anti-EphA2 antibody
Applications Tested/Suitable for anti-EphA2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-EphA2 antibody
Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:100~200.
Recommended starting dilution for Western blot is 1:500 and Immunohistochemistry is 1:100.

Western Blot (WB) of anti-EphA2 antibody
The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EphA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: EphA2 extracellular domain transfected 293T cell lysate Lane 3.: EphA2 cytoplasmic domain transfected 293T cell lysate
anti-EphA2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-EphA2 antibody
Paraffin embedded sections of human colon and gastric cancer tissue were incubated with anti-human EphA2 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
anti-EphA2 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for EphA2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
54,305 Da
NCBI Official Full Name
ephrin type-A receptor 2 isoform 1
NCBI Official Synonym Full Names
EPH receptor A2
NCBI Official Symbol
EPHA2  [Similar Products]
NCBI Official Synonym Symbols
ECK; CTPA; ARCC2; CTPP1; CTRCT6
  [Similar Products]
NCBI Protein Information
ephrin type-A receptor 2
UniProt Protein Name
Ephrin type-A receptor 2
UniProt Synonym Protein Names
Epithelial cell kinase; Tyrosine-protein kinase receptor ECK
Protein Family
UniProt Gene Name
EPHA2  [Similar Products]
UniProt Synonym Gene Names
NCBI Summary for EphA2
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]
UniProt Comments for EphA2
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
Product References and Citations for anti-EphA2 antibody
Ireton RC, Chen J: (2005) Curr Cancer Drug Tarqets. 5 (3), 149-57; Nakamoto M, Berqemann AD: (2002) Microsc Res Tech. 59 (1), 58-67

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000