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anti-HtrA2/Omi antibody :: Mouse anti-Human, Mouse HtrA2/Omi Monoclonal Antibody

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Catalog # MBS200025
Unit / Price
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  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Western Blot (WB)
Product Name

HtrA2/Omi, Monoclonal Antibody

Full Product Name

HtrA2/Omi antibody

Product Synonym Names
High temperature requirement protein A2; HTRA2; OMI; PRSS25; HtrA2/Omi; EC 3.4.21.108; High temperature requirement protein A2; HtrA2; Omi stress-regulated endoprotease; Serine proteinase OMI; Serine protease 25; Serine protease HTRA2; mitochondrial; High temperature requirement protein A2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
AF020760 mRNA
3D Structure
ModBase 3D Structure for O43464
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
1B3
Host
Mouse
Species Reactivity
Human, Mouse
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human HtrA2/Omi (134-458aa) purified from E. coli
Clone Note
Anti-human HtrA2/Omi mAb, clone 1B3, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HtrA2/Omi protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-HtrA2/Omi antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HtrA2/Omi antibody
HtrA2/Omi is a mammalian serine protease at high temperatures and a chaperone at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death
Product Categories/Family for anti-HtrA2/Omi antibody
Applications Tested/Suitable for anti-HtrA2/Omi antibody
ELISA (EIA), Western Blot (WB) (Cell lysate)
Application Notes for anti-HtrA2/Omi antibody
WB: 1:500 ~ 2,000.
Recommended starting dilution is 1:1,000.

Western Blot (WB) of anti-HtrA2/Omi antibody
The extracts of 293T, MCF7, HT-29, HL-60, HeLa, mouse brain, and rat brain were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HtrA2/Omi antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
anti-HtrA2/Omi antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for HtrA2/Omi. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
39,914 Da
NCBI Official Full Name
serine protease HTRA2, mitochondrial isoform 1 preproprotein
NCBI Official Synonym Full Names
HtrA serine peptidase 2
NCBI Official Symbol
HTRA2  [Similar Products]
NCBI Official Synonym Symbols
OMI; MGCA8; PARK13; PRSS25
  [Similar Products]
NCBI Protein Information
serine protease HTRA2, mitochondrial
UniProt Protein Name
Serine protease HTRA2, mitochondrial
UniProt Synonym Protein Names
High temperature requirement protein A2; HtrA2; Omi stress-regulated endoprotease; Serine protease 25; Serine proteinase OMI
UniProt Gene Name
HTRA2  [Similar Products]
UniProt Synonym Gene Names
OMI; PRSS25; HtrA2  [Similar Products]
NCBI Summary for HtrA2/Omi
This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
UniProt Comments for HtrA2/Omi
Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.
Product References and Citations for anti-HtrA2/Omi antibody
Miller L., et al., (1999) Trends Cell Biol. 9:323-28; Zumbrunn J., et al., (1996) FABS Lett. 398:187-92; Gui-Ying N., et al., (2003) Biochem. J. 371:39-48

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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