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anti-jak1 antibody :: Mouse anti-Human, Mouse JAK1 Monoclonal Antibody

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Catalog # MBS608588
Unit / Price
  0.1 mg  /  $630 +1 FREE 8GB USB
anti-jak1 antibody
Product Name

JAK1, Monoclonal Antibody

Also Known As

JAK1 (Janus Kinase 1)

Product Synonym Names
Anti -JAK1 (Janus Kinase 1)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 6; NC_007117.5 (31060241..31118556)
3D Structure
ModBase 3D Structure for O12990
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
10F612
Host
Mouse
Species Reactivity
Human, Mouse
Specificity
Recognizes endogenous human Jak1 at ~100-120kD.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, ph 7.4, 5% trehalose. Reconstitute with 200ul of sterile PBS.
Immunogen
Recombinant protein corresponding to aa32-286 of human Jak1 expressed in E. coli (P23458).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-jak1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-jak1 antibody
Janus Kinase 1 (Jak1) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of Jak1 occurs via phosphorylation at two adjacent tyrosine residues, Y1022 and Y1023, within the kinase domain. Jaks activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. Jak1 is required for the activation of STAT1 and STAT2 in response to IFN-alpha.
Applications Tested/Suitable for anti-jak1 antibody
Western Blot (WB)
Application Notes for anti-jak1 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 1ug/ml detects JAK1 on NIH-3T3 mouse embryonic fibroblast cell line, Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line and LNCaP human prostate cancer cell line.
NCBI/Uniprot data below describe general gene information for jak1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
132,481 Da[Similar Products]
NCBI Official Full Name
jak1
NCBI Official Synonym Full Names
Janus kinase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
tyrosine-protein kinase JAK1; jak-1; jak1 kinase; etID309794.6
UniProt Protein Name
Tyrosine-protein kinase JAK1
UniProt Synonym Protein Names
Janus kinase 1
Protein Family
UniProt Gene Name
UniProt Entry Name
JAK1_DANRE
UniProt Comments for jak1
Function: Tyrosine kinase of the non-receptor type. Appears to be required in early development for specific cell migrations (epiboly), expression of homeobox protein goosecoid and formation of anterior structures. Ref.1

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location: Endomembrane system; Peripheral membrane protein

By similarity. Note: Wholly intracellular, possibly membrane associated

Developmental stage: Present in the unfertilized egg through to the blastula stage where it is distributed uniformly. Levels drop rapidly at four hours development, remain very low until 10 hours, then gradually increase from 12 hours with a rapid increase at 48 hours. At 48 hours it is concentrated in the region of the gill arches. Also present in the adult. Ref.1

Domain: Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.Contains 1 FERM domain.Contains 1 protein kinase domain.Contains 1 SH2 domain.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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