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anti-MAPK14 antibody :: Rabbit anti-Human, Mouse MAP Kinase, p38 alpha Monoclonal Antibody

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Catalog # MBS609021
Unit / Price
  0.1 mL  /  $615 +1 FREE 8GB USB
anti-MAPK14 antibody
Product Name

MAP Kinase, p38 alpha (MAPK14), Monoclonal Antibody

Also Known As

MAP Kinase, p38 alpha, phosphorylated (Thr180, Ty182) (Mitogen-activated Protein Kinase p38 alpha, MAPK p38 alpha, Cytokine Suppressive Anti-inflammatory Drug-binding Protein, CSAID-binding Protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP Kinase MXI2, MAX-in

Product Synonym Names
Anti -MAP Kinase, p38 alpha, phosphorylated (Thr180, Ty182) (Mitogen-activated Protein Kinase p38 alpha, MAPK p38 alpha, Cytokine Suppressive Anti-inflammatory Drug-binding Protein, CSAID-binding Protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP Kinase MXI2, MAX-in
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 6; NC_000006.11 (35995412..36079013). Location: 6p21.3-p21.2
OMIM
600289
3D Structure
ModBase 3D Structure for Q16539
Clonality
Monoclonal
Isotype
IgG
Clone Number
9H254
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
Recognizes human p38 alpha when phosphorylated on Thr180/Tyr182. Species Crossreactivity: mouse. Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
Purity/Purification
Supernatant
Supernatant
Form/Format
Supplied as a liquid, 0.05% sodium azide.
Immunogen
Synthetic peptide corresponding to amino acids surrounding and including phosphorylated Thr180/Tyr182 of human p38 alpha.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-MAPK14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MAPK14 antibody
The p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation.
Applications Tested/Suitable for anti-MAPK14 antibody
Western Blot (WB)
Application Notes for anti-MAPK14 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 1:5000-1:10000
NCBI/Uniprot data below describe general gene information for MAPK14. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
41,293 Da[Similar Products]
NCBI Official Full Name
mitogen-activated protein kinase 14 isoform 4
NCBI Official Synonym Full Names
mitogen-activated protein kinase 14
NCBI Official Symbol
MAPK14  [Similar Products]
NCBI Official Synonym Symbols
RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
  [Similar Products]
NCBI Protein Information
mitogen-activated protein kinase 14; MAP kinase 14; p38alpha Exip; p38 MAP kinase; MAP kinase Mxi2; MAP kinase p38 alpha; CSAID-binding protein; Csaids binding protein; MAX-interacting protein 2; stress-activated protein kinase 2A; p38 mitogen activated protein kinase; mitogen-activated protein kinase p38 alpha; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug-binding protein
UniProt Protein Name
Mitogen-activated protein kinase 14
UniProt Synonym Protein Names
Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Stress-activated protein kinase 2a
UniProt Gene Name
MAPK14  [Similar Products]
UniProt Synonym Gene Names
CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; MAP kinase 14; MAPK 14; CSAID-binding protein; CSBP; MAP kinase p38 alpha; SAPK2a  [Similar Products]
UniProt Entry Name
MK14_HUMAN
NCBI Summary for MAPK14
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Comments for MAPK14
P38A: a proline-directed ser/thr MAP kinase, and one of four p38 kinases that play important roles in cellular responses to inflammatory cytokines, DNA damage, oxidative stress, and some GPCRs, leading to direct activation of transcription factors and of other downstream kinases including MSK1, MSK2, eEF2K, MK2, and PRAK. MSK1 and -2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli. MK2 and -3 control gene expression mostly at the post-transcriptional level. eEF2K is important for the elongation of mRNA during translation. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17, which then cleaves the ectodomain of TGF-alpha family ligands, a process leading to the activation of EGFR signaling and cell proliferation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, CHOPO, p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. Interacts directly with HDAC3 interacts directly and selectively to repress ATF2 transcriptional activity, and regulate TNF gene expression in LPS-stimulated cells. Phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. May also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Regulates the endocytosis of membrane receptors that depend on RAB5A. Regulates the clathrin-mediated internalization of EGFR induced by inflammatory cytokines and UV irradiation by phosphorylating the EGFR and RAB5A effectors. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B. Activated by cell stresses such as DNA damage, heat shock, osmotic shock, anisomycin and sodium arsenite, as well as pro-inflammatory stimuli such as LPS and IL-1. Phosphorylated by ZAP70 in an alternative activation pathway in response to TCR signaling in T-cells, a pathway is inhibited by GADD45A. Four alternatively spliced isoforms of the human protein have been observed. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis

Protein type: EC 2.7.11.24; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; MAPK/p38 subfamily; p38 subfamily

Chromosomal Location of Human Ortholog: 6p21.3-p21.2

Cellular Component: nucleoplasm; spindle pole; mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: MAP kinase kinase activity; MAP kinase activity; protein serine/threonine kinase activity; protein binding; NFAT protein binding; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; stress-activated MAPK cascade; osteoclast differentiation; toll-like receptor 3 signaling pathway; toll-like receptor 5 signaling pathway; cell surface receptor linked signal transduction; regulation of transcription factor activity; transmembrane receptor protein serine/threonine kinase signaling pathway; chondrocyte differentiation; toll-like receptor 4 signaling pathway; cartilage condensation; mitochondrion organization and biogenesis; platelet activation; skeletal muscle development; transcription, DNA-dependent; positive regulation of blood vessel endothelial cell migration; glucose metabolic process; toll-like receptor 2 signaling pathway; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; response to muramyl dipeptide; DNA damage checkpoint; striated muscle cell differentiation; fatty acid oxidation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; myoblast cell differentiation involved in skeletal muscle regeneration; apoptosis; cell morphogenesis; chemotaxis; signal transduction; toll-like receptor 10 signaling pathway; lipopolysaccharide-mediated signaling pathway; angiogenesis; MyD88-independent toll-like receptor signaling pathway; organelle organization and biogenesis; positive regulation of erythrocyte differentiation; DNA damage response, signal transduction; positive regulation of myoblast differentiation; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; positive regulation of protein import into nucleus; Ras protein signal transduction; toll-like receptor signaling pathway; positive regulation of muscle cell differentiation; innate immune response; gene expression; cell motility; blood coagulation; vascular endothelial growth factor receptor signaling pathway
Precautions
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Disclaimer
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