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anti-ACTN1 antibody :: Mouse Actinin, alpha 1 Monoclonal Antibody

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Catalog # MBS600408
Unit / Price
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  0.2 mg  /  $715 +1 FREE 8GB USB
anti-ACTN1 antibody
Product Name

Actinin, alpha 1 (ACTN1), Monoclonal Antibody

Full Product Name

Actinin, alpha 1 (Non-muscle)

Product Synonym Names
Anti -Actinin, alpha 1 (Non-muscle)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 14; NC_000014.8 (69340840..69446083, complement). Location: 14q24.1-q24.2; 14q24; 14q22-q24
OMIM
102575
3D Structure
ModBase 3D Structure for P12814
Clonality
Monoclonal
Isotype
IgG1
Clone Number
0.T.02
Host
Mouse
Species Reactivity
Human, Mouse, Rabbit
Specificity
Recognizes human alpha-Actinin 1 isoform (non-muscle) at 100kD. Species Crossreactivity: mouse and rabbit
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Immunogen
Human alpha-Actinin
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-ACTN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACTN1 antibody
The cytoplasmic cytoskeleton determines cell organization, shape and adhesion among other functions. Furthermore, the cell type-specific expression of intermediate filaments allows determination of the origin of many, otherwise unspecific tumors.
Applications Tested/Suitable for anti-ACTN1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for anti-ACTN1 antibody
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Not suitable for use in Immunoprecipitation.
Dilution: Western Blot: 0.5-2ug/ml. Detects alpha-Actinin in RIPA lysates from human A431 cells.
Immunocytochemistry/Immunohistochemistry: 15ug/ml. Reported positive immunostaining for alpha-Actinin in MRC-5 cells fixed with methanol and also in frozen sections.
NCBI/Uniprot data below describe general gene information for ACTN1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
103,058 Da[Similar Products]
NCBI Official Full Name
actinin, alpha 1
NCBI Official Synonym Full Names
actinin, alpha 1
NCBI Official Symbol
ACTN1  [Similar Products]
NCBI Official Synonym Symbols
FLJ40884; FLJ54432
  [Similar Products]
NCBI Protein Information
alpha-actinin-1; actinin 1 smooth muscle; non-muscle alpha-actinin-1; F-actin cross-linking protein; alpha-actinin cytoskeletal isoform
UniProt Protein Name
Alpha-actinin-1
UniProt Synonym Protein Names
Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1
Protein Family
UniProt Gene Name
ACTN1  [Similar Products]
UniProt Entry Name
ACTN1_HUMAN
NCBI Summary for ACTN1
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
UniProt Comments for ACTN1
ACTN1: a cytoskeletal protein of the spectrin superfamily. An actin-binding protein with multiple roles in different cell types. A nonmuscle isoform structurally similar to the erythroid beta spectrin gene.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 14q24|14q22-q24

Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; extracellular region; dendritic spine; pseudopodium; fascia adherens; Z disc; cytosol; ruffle; cell projection; cytoplasm; plasma membrane; stress fiber; nucleus

Molecular Function: integrin binding; actin filament binding; protein domain specific binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; double-stranded RNA binding; calcium ion binding; vinculin binding

Biological Process: actin crosslink formation; focal adhesion formation; regulation of apoptosis; actin filament bundle formation; platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; negative regulation of cell motility; blood coagulation

Disease: Bleeding Disorder, Platelet-type, 15
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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