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anti-YWHAB antibody :: Mouse 14-3-3 Beta/Alpha Monoclonal Antibody

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Catalog # MBS225457
Unit / Price
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  0.1 mL  /  $425 +1 FREE 8GB USB
Western Blot (WB)
Product Name

14-3-3 Beta/Alpha (YWHAB), Monoclonal Antibody

Full Product Name

14-3-3 Beta/Alpha Antibody with Control Lysate

Product Synonym Names
Mouse anti 14-3-3 BETA/ALPHA; 14-3-3 Beta/Alpha Antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
601289
3D Structure
ModBase 3D Structure for P31946
Clonality
Monoclonal
Isotype
IgG2a
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Purified by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Immunogen
Recombinant human 14-3-3 beta/alpha
Lysate Composition
400ug MCF7 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
Preparation and Storage
Antibody: Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Other Notes
Small volumes of anti-YWHAB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-YWHAB antibody
Western Blot (WB)
Application Notes for anti-YWHAB antibody
WB: 1:1000
Western Blotting: Detects a band of approximately 28 kDa in MCF7 cell lysates.

Western Blot (WB) of anti-YWHAB antibody
Western blot analysis of whole cell lysates probed with 14-3-3 beta/alpha antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 82.1 second exposure. Arrow points to 14-3-3 beta/alpha (molecular weight 28 kDa)
anti-YWHAB antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for YWHAB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
28 kDa
NCBI Official Full Name
14-3-3 protein beta/alpha
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta
NCBI Official Symbol
YWHAB  [Similar Products]
NCBI Official Synonym Symbols
HS1; GW128; YWHAA; KCIP-1; HEL-S-1
  [Similar Products]
NCBI Protein Information
14-3-3 protein beta/alpha
UniProt Protein Name
14-3-3 protein beta/alpha
UniProt Synonym Protein Names
Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1
Protein Family
UniProt Gene Name
YWHAB  [Similar Products]
UniProt Synonym Gene Names
KCIP-1  [Similar Products]
NCBI Summary for YWHAB
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for YWHAB
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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