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anti-APAF1 antibody :: Mouse Apaf-1 Monoclonal Antibody

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Catalog # MBS150040
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

Apaf-1 (APAF1), Monoclonal Antibody

Also Known As

Apaf-1 Antibody [2E10]

Product Synonym Names
Apaf-1 Antibody [2E10] : CED4; APAF-1; apoptotic peptidase activating factor 1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1194
OMIM
602233
3D Structure
ModBase 3D Structure for O14727
Clonality
Monoclonal
Clone Number
2E10
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Apaf-1 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
A peptide corresponding to amino acids near the carboxy terminus of human Apaf1.
Buffer
Apaf-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
Apaf-1 monoclonal antibody can be stored at -20 degree C, stable for one year.
Other Notes
Small volumes of anti-APAF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-APAF1 antibody
Apaf-1 Monoclonal Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.
Applications Tested/Suitable for anti-APAF1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-APAF1 antibody
Apaf1 antibody can be used for detection of Apaf1 by Western blot at 0.25 - 0.5 mg/mL. A 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 0.5 mug/mL.

Western Blot (WB) of anti-APAF1 antibody
Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 0.25 and (B) 0.5 mg/mL.
anti-APAF1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-APAF1 antibody
Immunohistochemistry of Apaf1 in K562 cells with Apaf1 antibody at 0.5 μg/mL.
anti-APAF1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for APAF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
130 kDa
NCBI Official Full Name
apoptotic protease activating factor 1
NCBI Official Synonym Full Names
apoptotic peptidase activating factor 1
NCBI Official Symbol
APAF1  [Similar Products]
NCBI Official Synonym Symbols
CED4; APAF-1
  [Similar Products]
NCBI Protein Information
apoptotic protease-activating factor 1
UniProt Protein Name
Apoptotic protease-activating factor 1
UniProt Gene Name
APAF1  [Similar Products]
UniProt Synonym Gene Names
KIAA0413; APAF-1  [Similar Products]
UniProt Entry Name
APAF_HUMAN
NCBI Summary for APAF1
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Comments for APAF1
APAF: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). By E2F and p53/TP53 in apoptotic neurons. Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 12q23

Cellular Component: nucleus; cytosol; apoptosome

Molecular Function: identical protein binding; protein binding; nucleotide binding; caspase activator activity; ADP binding; ATP binding

Biological Process: caspase activation; regulation of apoptosis; nervous system development; neuron apoptosis; positive regulation of apoptosis; apoptosis; forebrain development; neural tube closure; caspase activation via cytochrome c
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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