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anti-b-Actin antibody :: Rabbit b-Actin Monoclonal Antibody

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Catalog # MBS684073
Unit / Price
  0.04 mL  /  $185 +1 FREE 8GB USB
  0.1 mL  /  $270 +1 FREE 8GB USB
  0.2 mL  /  $335 +1 FREE 8GB USB
  0.5 mL  /  $415 +1 FREE 8GB USB
  1 mL  /  $615 +1 FREE 8GB USB
Testing Data
Product Name

b-Actin, Monoclonal Antibody

Also Known As

Anti - b-Actin

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
375
Chromosome Location
Chromosome: 7; NC_000007.14 (5527148..5530601, complement). Location: 7p22
OMIM
102630
3D Structure
ModBase 3D Structure for P60709
Clonality
Monoclonal
Clone Number
S12-I
Host
Rabbit
Species Reactivity
Human, mouse, rat
Specificity
Human
Immunogen
Peptide derived from C-terminal sequence of human beta-actin
Storage Buffer
20 mM Tris-HCl, pH 8.0
Stabilizer
20 mg/ml BSA
Preservative
0.05% NaN3
Preparation and Storage
Store at +4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Other Notes
Small volumes of anti-b-Actin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-b-Actin antibody
Immunohistochemistry (IHC) - Formalin/Paraffin
Application Notes for anti-b-Actin antibody
IHC-P dilution 1:100 - 1:200; IHC-Fr application and dilution to be tested by the user

Testing Data of anti-b-Actin antibody
anti-b-Actin antibody Testing Data image
NCBI/Uniprot data below describe general gene information for b-Actin. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
41,737 Da
NCBI Official Full Name
Actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
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NCBI Protein Information
actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Synonym Protein Names
Beta-actin
UniProt Gene Name
UniProt Entry Name
ACTB_HUMAN
NCBI Summary for b-Actin
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]
UniProt Comments for b-Actin
Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subunit structure: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of KMT2E/MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCSAM. Ref.11 Ref.12 Ref.14 Ref.15 Ref.16 Ref.18 Ref.21

Subcellular location: Cytoplasm › cytoskeleton. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Ref.15

Post-translational modification: ISGylated. Ref.13Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization

By similarity.Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.

Involvement in disease: Dystonia, juvenile-onset (DYTJ) [MIM:607371]: A form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. Patients with juvenile-onset dystonia manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.20Baraitser-Winter syndrome 1 (BRWS1) [MIM:243310]: A rare developmental disorder characterized by the combination of congenital ptosis, high-arched eyebrows, hypertelorism, ocular colobomata, and a brain malformation consisting of anterior-predominant lissencephaly. Other typical features include postnatal short stature and microcephaly, intellectual disability, seizures, and hearing loss.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.23

Miscellaneous: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Sequence similarities: Belongs to the actin family.
Research Articles on b-Actin
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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