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anti-KCNMB3 antibody :: Mouse BK Beta3a Monoclonal Antibody

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Catalog # MBS801301
Unit / Price
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  0.1 mg  /  $440 +1 FREE 8GB USB
Product Name

BK Beta3a (KCNMB3), Monoclonal Antibody

Full Product Name

BK Beta3a Antibody : ATTO 390

Product Synonym Names
HBETA3; KCNMB2; KCNMBL; BKBeta3; KCNMB3; Calcium-activated potassium channel subunit beta-3; BK channel subunit beta-3; BKbeta3; Hbeta3; Calcium-activated potassium channel; subfamily M subunit beta-3 Charybdotoxin receptor subunit beta-3; K(VCA)beta-3
Matching Pairs
Unconjugated Antibody: BK Beta3a Clone #S40B-18 (MBS801488)
ATTO 390 Conjugated Antibody: BK Beta3a Clone #S40B-18 (MBS801301)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
605222
3D Structure
ModBase 3D Structure for Q9NPA1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S40B-18
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Conjugate
ATTO 390
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Other Notes
Small volumes of anti-KCNMB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-KCNMB3 antibody
Background Info: Detects ~32 kDa. No cross-reactivity against BKBeta1, BKBeta2, BKBeta3b or BKBeta4.

Scientific Background: BK channels contribute to electrical impulses, proper signal transmission of information and regulation of neurotransmitter release (3). A gain of function mutation in the pore-forming alpha subunit of the BK channel was linked to human neurological diseases. Findings suggest that the distribution of the beta subunits in the brain can modulate the BK channels to contribute to the pathophysiology of epilepsy and dyskinesia (4). This has major implications on other physiological processes in tissues other than the brain.
Product Categories/Family for anti-KCNMB3 antibody
Applications Tested/Suitable for anti-KCNMB3 antibody
Western (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-KCNMB3 antibody
1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-KCNMB3 antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody, Clone S40B-18. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Accumulation at the edges of the cell, ruffling edges.
anti-KCNMB3 antibody Immunocytochemistry/Immunofluorescence (ICC/IF) (ICC) image
Western Blot (WB) of anti-KCNMB3 antibody
Western Blot analysis of Rat brain membrane lysate showing detection of KCNMB3 Potassium Channel protein using Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody, Clone S40B-18. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
anti-KCNMB3 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-KCNMB3 antibody
Immunohistochemistry analysis using Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody, Clone S40B-18. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Beautiful filaggrin-like staining.
anti-KCNMB3 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for KCNMB3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
19,474 Da[Similar Products]
NCBI Official Full Name
calcium-activated potassium channel subunit beta-3 isoform d
NCBI Official Synonym Full Names
potassium large conductance calcium-activated channel, subfamily M beta member 3
NCBI Official Symbol
KCNMB3  [Similar Products]
NCBI Official Synonym Symbols
HBETA3; KCNMB2; KCNMBL; BKBETA3; SLOBETA3; SLO-BETA-3; K(VCA)BETA-3
  [Similar Products]
NCBI Protein Information
calcium-activated potassium channel subunit beta-3; BK channel subunit beta-3; maxi K channel subunit beta-3; charybdotoxin receptor subunit beta-3; calcium-activated potassium channel regulatory subunit; calcium-activated potassium channel, subfamily M subunit beta-3; large conductance, voltage and Ca2+ activated potassium channel Maxi K beta 3 subunit
UniProt Protein Name
Calcium-activated potassium channel subunit beta-3
UniProt Synonym Protein Names
BK channel subunit beta-3; BKbeta3; Hbeta3; Calcium-activated potassium channel, subfamily M subunit beta-3; Charybdotoxin receptor subunit beta-3; K(VCA)beta-3; Maxi K channel subunit beta-3; Slo-beta-3
UniProt Gene Name
KCNMB3  [Similar Products]
UniProt Synonym Gene Names
KCNMB2; KCNMBL; BKbeta3; Hbeta3  [Similar Products]
UniProt Entry Name
KCMB3_HUMAN
NCBI Summary for KCNMB3
MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. The protein encoded by this gene is an auxiliary beta subunit which may partially inactivate or slightly decrease the activation time of MaxiK alpha subunit currents. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 22. [provided by RefSeq, Jul 2009]
UniProt Comments for KCNMB3
KCNMB3: Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Alters the functional properties of the current expressed by the KCNMA1 channel. Isoform 2, isoform 3 and isoform 4 partially inactivate the current of KCNBMA. Isoform 4 induces a fast and incomplete inactivation of KCNMA1 channel that is detectable only at large depolarizations. In contrast, isoform 1 does not induce detectable inactivation of KCNMA1. Two or more subunits of KCNMB3 are required to block the KCNMA1 tetramer. Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB3 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q26.3-q27

Cellular Component: voltage-gated potassium channel complex; integral to plasma membrane; plasma membrane

Molecular Function: calcium-activated potassium channel activity; potassium channel regulator activity

Biological Process: regulation of action potential; synaptic transmission; generation of action potential; detection of calcium ion; blood coagulation; potassium ion transport
Product References and Citations for anti-KCNMB3 antibody
1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Wulf-Johansson H., et al. (2009) Brain Res. 1292: 1- 13. 4. Lee U.S., and Cui J. (2009) J Physiol. 587(7): 1481- 1489.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with anti-KCNMB3 antibody
Diseases associated with anti-KCNMB3 antibody
Organs/Tissues associated with anti-KCNMB3 antibody
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