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anti-CBL antibody :: Mouse c-Cbl (C-terminal Region) Monoclonal Antibody

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Catalog # MBS470005
Unit / Price
  0.1 mL  /  $280 +1 FREE 8GB USB
Testing Data
Product Name

c-Cbl (C-terminal Region), Monoclonal Antibody

Full Product Name

Anti-c-Cbl (C-terminal Region)

Product Synonym Names
c-Cbl (C-terminal Region); E3 ubiquitin-protein ligase CBL; RING finger protein 55
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 11; NC_000011.9 (119076990..119178859). Location: 11q23.3
OMIM
165360
3D Structure
ModBase 3D Structure for P22681
Clonality
Monoclonal
Isotype
IgG1
Host
Mouse
Species Reactivity
Human, Rat, Mouse
Specificity
The antibody detects a 120 kDa* protein in human Jurkat cells.
Purity/Purification
Protein A Purified
Form/Format
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Immunogen
Clone (M159) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of c-Cbl. This sequence is highly conserved in rat and mouse c-Cbl.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Other Notes
Small volumes of anti-CBL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CBL antibody
c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells. c-Cbl possesses a highly conserved amino-terminal phosphotyrosine binding domain and a C3HC4 RING finger motif. c-Cbl is rapidly tyrosine phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules such as protein tyrosine kinases, phosphatidylinositol 3 kinase (PI3K), Crk and 14-3-3 proteins. In human cancer tissues, c-Cbl is frequently tyrosine phosphorylated in a tumor-specific manner. Src-family kinases primarily phosphorylate c- Cbl at Tyr-700, Tyr-731, and Tyr-774. These sites of phosphorylation provide docking sites for downstream signaling components, such as Grb-2, PI3K, and Fyn.
Applications Tested/Suitable for anti-CBL antibody
ELISA, Western Blot
Application Notes for anti-CBL antibody
ELISA: 1:2000
Western Blot: 1:1000

Testing Data of anti-CBL antibody
anti-CBL antibody Testing Data image
NCBI/Uniprot data below describe general gene information for CBL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
99,633 Da[Similar Products]
NCBI Official Full Name
c-cbl
NCBI Official Synonym Full Names
Cas-Br-M (murine) ecotropic retroviral transforming sequence
NCBI Official Symbol
NCBI Official Synonym Symbols
CBL2; NSLL; C-CBL; RNF55
  [Similar Products]
NCBI Protein Information
E3 ubiquitin-protein ligase CBL; oncogene CBL2; OTTHUMP00000232087; proto-oncogene c-Cbl; RING finger protein 55; signal transduction protein CBL; casitas B-lineage lymphoma proto-oncogene
UniProt Protein Name
E3 ubiquitin-protein ligase CBL
UniProt Synonym Protein Names
Casitas B-lineage lymphoma proto-oncogene; Proto-oncogene c-Cbl; RING finger protein 55; Signal transduction protein CBL
UniProt Gene Name
UniProt Synonym Gene Names
CBL2; RNF55  [Similar Products]
UniProt Entry Name
CBL_HUMAN
NCBI Summary for CBL
The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation. [provided by RefSeq]
UniProt Comments for CBL
Function: Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling. Essential for osteoclastic bone resorption. The Tyr-731 phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Ref.4 Ref.17

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Associates with NCK via its SH3 domain. The phosphorylated C-terminus interacts with CD2AP via its second SH3 domain. Binds to UBE2L3. Interacts with adapters SLA, SLA2 and with the phosphorylated C-terminus of SH2B2. Interacts with EGFR, SYK and ZAP70 via the highly conserved Cbl-N region. Also interacts with SORBS1 and INPPL1/SHIP2. Interacts with phosphorylated LAT2. May interact with CBLB

By similarity. Ref.6 Ref.8 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16

Subcellular location: Cytoplasm.

Domain: The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.

Post-translational modification: Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70

By similarity. Phosphorylated on tyrosine residues by INSR. Ref.5 Ref.7 Ref.9 Ref.15 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24

Involvement in disease: Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [

MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects. Ref.29

Miscellaneous: This protein has one functional calcium-binding site.

Sequence similarities: Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.Contains 1 RING-type zinc finger.Contains 1 UBA domain.
Product References and Citations for anti-CBL antibody
• Blake, T. J. et al. (1991) Oncogene 6:653.
• Donovan, J.A., et al. (1994) J. Biol. Chem. 269:37.
• Rivero-Lezcano, O.M. et al. (1994) J. Biol. Chem. 269:26.
• Christine, B. F. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:294.

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Disclaimer
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