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anti-c-erbB-2/HER-2 antibody :: Mouse c-erbB-2/HER-2 Monoclonal Antibody

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Catalog # MBS462228
Unit / Price
  0.1 mg  /  $285 +1 FREE 8GB USB
Testing Data
Product Name

c-erbB-2/HER-2, Monoclonal Antibody

Also Known As

Mouse anti c-erbB-2/HER-2

Product Synonym Names
Ms anti-c-erbB-2/HER-2; human epidermal growth factor Receptor 2 (Her-2); p185erbB2; MLN19; C-erbB-2; NEU proto-oncogene; Tyrosine kinase-type cell surface receptor HER2; CD340 antigen
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
SPM495
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes Her-2 protein. It cross-reacts with human, rat and mouse. The other species was not tested.
Purity/Purification
The Mouse IgG is purified by Isotyping-specific Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (lot specific)
Antigen Preparation
A synthetic peptide corresponding to the C-terminus of Her2 protein. This sequence is identical in human, mouse, and rat.
Positive Control
SK-BR-3 cell lysate
Cellular Location
Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Other Notes
Small volumes of anti-c-erbB-2/HER-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-c-erbB-2/HER-2 antibody
Oncoprotein Her-2/Neu/ c-erbB-2 belongs to one of the four members of the ErbB receptor family. They are transmembrane receptor-like tyrosine kinases which can be autophosphorylated without ligand binding process when it is overexpressed in breast cancers. Her2 is an important biomarker in breast cancer diagnosis. The antibodies/ heregulin can suppress phosphorylation of HER2 on tyrosine Y1248. Endogenous anti-HER2 antibodies can effectively suppress HER2 kinase activity and downstream signaling to inhibit the transformed phenotype of HER2-expressing tumor cells. Thus, anti-Her2 antibodies have been used widely in the treatment of the breast cancer and other carcinomas.
Applications Tested/Suitable for anti-c-erbB-2/HER-2 antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes for anti-c-erbB-2/HER-2 antibody
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
Flow Cytometry: 5-10 ug/ml

Molecular Weight
~185 kDa
Product References and Citations for anti-c-erbB-2/HER-2 antibody
Xiao-Feng Le et al (2000) Anti-HER2 Antibody and Heregulin Suppress Growth of HER2-Overexpressing Human Breast Cancer Cells through Different Mechanisms. Clinical Cancer Research Vol. 6, 260-270.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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