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anti-CACNA1H antibody :: Mouse Cav3.2 Monoclonal Antibody

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Catalog # MBS803808
Unit / Price
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  0.1 mg  /  $425 +1 FREE 8GB USB
Product Name

Cav3.2 (CACNA1H), Monoclonal Antibody

Popular Item
Full Product Name

CaV3.2 Antibody: HRP

Product Synonym Names
Cav3.2; CACNA1H; CACNA1HB; calcium channel; voltage-dependent; T type; alpha 1H subunit; calcium channel; voltage-dependent; T type; alpha 1Hb subunit; ECA6; EIG6; FLJ90484; Low-voltage-activated calcium channel alpha1 3.2 subunit
Matching Pairs
Unconjugated Antibody: Cav3.2 Clone #S55-10 (MBS800902)
HRP Conjugated Antibody: Cav3.2 Clone #S55-10 (MBS803808)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
gene 611942
3D Structure
ModBase 3D Structure for O95180
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S55-10
Host
Mouse
Species Reactivity
Human, Mouse, Rat.
Specificity
Detects ~260kDa. No cross-reactivity against Cav1.3
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Conjugate
HRP
Immmunogen
Fusion protein amino acids 1019-1293 (II-III loop} of human Cav3.2
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Certificate of Analysis
1 ug/ml of SMC-303 was sufficient for detection of Cav3.2 in 10ug of HEK cell lysate expressing Cav3.2 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.
Research Area(s)
Neuroscience
Ion Channels
Calcium Channels
Voltage-Gated Calcium Channels
Cancer
Cell Signaling
Cellular Localization
Membrane
Preparation and Storage
-20 degree C; 1 year +
Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-CACNA1H antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CACNA1H antibody
Background Info: Detects ~260kDa. No cross-reactivity against Cav1.3 or Cav3.1

Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand-gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, CaV3.2 is a protein which in humans is encoded by the CACNA1H gene. Studies suggest certain mutations in this gene lead to childhood absence epilepsy (3, 4). Studies also suggest that the up-regulations of CaV3.2 may participate in the progression of prostate cancer toward an androgen-independent stage (5).
Product Categories/Family for anti-CACNA1H antibody
Applications Tested/Suitable for anti-CACNA1H antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-CACNA1H antibody
WB (1:1000), IHC (1:1000), ICC/IF (1:100); Optimal dilutions for assays should be determined by the user.

Immunohistochemistry (IHC) of anti-CACNA1H antibody
Immunohistochemistry analysis using Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody, Clone S55-10. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV3.2 Calcium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
anti-CACNA1H antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CACNA1H antibody
Immunohistochemistry analysis using Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody, Clone S55-10. Tissue: frozen brain section. Species: human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-CaV3.2 Calcium channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.
anti-CACNA1H antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CACNA1H. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
258,545 Da[Similar Products]
NCBI Official Full Name
voltage-dependent T-type calcium channel subunit alpha-1H isoform b
NCBI Official Synonym Full Names
calcium channel, voltage-dependent, T type, alpha 1H subunit
NCBI Official Symbol
CACNA1H  [Similar Products]
NCBI Official Synonym Symbols
ECA6; EIG6; Cav3.2; CACNA1HB
  [Similar Products]
NCBI Protein Information
voltage-dependent T-type calcium channel subunit alpha-1H; voltage-gated calcium channel alpha subunit Cav3.2; voltage-gated calcium channel alpha subunit CavT.2; voltage-gated calcium channel subunit alpha Cav3.2; low-voltage-activated calcium channel alpha13.2 subunit; low-voltage-activated calcium channel alpha1 3.2 subunit; voltage dependent t-type calcium channel alpha-1H subunit; calcium channel, voltage-dependent, T type, alpha 1Hb subunit
UniProt Protein Name
Voltage-dependent T-type calcium channel subunit alpha-1H
UniProt Synonym Protein Names
Low-voltage-activated calcium channel alpha1 3.2 subunit; Voltage-gated calcium channel subunit alpha Cav3.2
UniProt Gene Name
CACNA1H  [Similar Products]
UniProt Entry Name
CAC1H_HUMAN
NCBI Summary for CACNA1H
This gene encodes a T-type member of the alpha-1 subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. The alpha-1 subunit has 24 transmembrane segments and forms the pore through which ions pass into the cell. There are multiple isoforms of each of the proteins in the complex, either encoded by different genes or the result of alternative splicing of transcripts. Alternate transcriptional splice variants, encoding different isoforms, have been characterized for the gene described here. Studies suggest certain mutations in this gene lead to childhood absence epilepsy (CAE). [provided by RefSeq, Jul 2008]
UniProt Comments for CACNA1H
CACNA1H: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1H gives rise to T-type calcium currents. T-type calcium channels belong to the low-voltage activated (LVA) group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes. Defects in CACNA1H are a cause of susceptibility to epilepsy, idiopathic generalized type 6 (EIG6). A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain. EIG6 is a polygenic and multifactorial disease. Defects in CACNA1H are a cause of susceptibility to epilepsy, childhood absence type 6 (ECA6). A subtype of idiopathic generalized epilepsy characterized by an onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. Tonic- clonic seizures often develop in adolescence. Absence seizures may either remit or persist into adulthood. Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1H subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Channel, calcium; Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: plasma membrane; integral to membrane; voltage-gated calcium channel complex; caveola; sarcolemma

Molecular Function: low voltage-gated calcium channel activity; metal ion binding

Biological Process: axon guidance; regulation of membrane potential; muscle development; muscle contraction; transport; aldosterone biosynthetic process; myoblast fusion; regulation of heart contraction; cellular response to hormone stimulus

Disease: Epilepsy, Childhood Absence, Susceptibility To, 6
Product References and Citations for anti-CACNA1H antibody
1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA. 2. www.iochannels.org 3. Chen Y., et al. (2003) Ann. Neurol. 54(2): 239-43. 4. Khosravani H., et al. (2004) J Biol Chem. 279(11): 9681- 9684. 5. Gackiere F., et al. (2008) J Biol Chem. 283(28): 19872.

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Disclaimer
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