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Mouse Flag-Tag Monoclonal Antibody

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Catalog # MBS9400453
Unit / Price
  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $255 +1 FREE 8GB USB
Product Name

Flag-Tag, Monoclonal Antibody

Popular Item
Also Known As

Flag-Tag Mouse Monoclonal Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity purification using immunogen.
Form/Format
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (lot specific)
Target Name
Flag-Tag
Specifity
The Flag tag antibody can recognize C-terminal, internal and N-terminal Flag-tag fusion proteins.
Preparation and Storage
Store at -20°C
Other Notes
Small volumes of anti-Flag-Tag antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Flag-Tag antibody
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
Product Categories/Family for anti-Flag-Tag antibody
Applications Tested/Suitable for anti-Flag-Tag antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-Flag-Tag antibody
Western blotting: 1:5000~1:10000
Immunofluorescence: 1:2000
Immunoprecipitation: 1:5000

Testing Data of anti-Flag-Tag antibody
1ug Flag fusion protein+ Primary antibody dilution at 1:10,000.
anti-Flag-Tag antibody Testing Data image
Immunofluorescence (IF) of anti-Flag-Tag antibody
IF analysis of 293 cells transfected with a Flag-tag protein, using anti-Flag-Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag).
anti-Flag-Tag antibody Immunofluorescence (IF) image
Immunoprecipitation (IP) of anti-Flag-Tag antibody
IP antibody use 15 ug Flag Mouse IgG1 per ml
Lysate WB 1:5000

1 B'B untransfected 293 cell lysate
2 B'B transfected 293 cell lysate with Flag-tag fusion protein
3 B'B'IP(transfected 293+ normal Mouse IgG+Protein G agarose)
4 B'B'IP (transfected 293+anti- Flag mAb+ Protein G agarose)
5 B'B'IP(transfected 293+Protein G)
6 B'B'Recombinant protein (E Coli).
anti-Flag-Tag antibody Immunoprecipitation (IP) (IP) image
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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