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anti-Grin2b antibody :: Mouse GluN2B/NR2B Monoclonal Antibody

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Catalog # MBS800075
Unit / Price
  0.1 mg  /  $390 +1 FREE 8GB USB
Product Name

GluN2B/NR2B (Grin2b), Monoclonal Antibody

Popular Item
Full Product Name

Mouse Anti-Rat GluN2B/NR2B Monoclonal IgG2b

Product Synonym Names
GluN2B/NR2B Antibody, 559-36; NMDAR2B; hNR3; MGC142180; glutamate receptor subunit epsilon-2; NR3
Matching Pairs
Unconjugated Antibody: GluN2B/NR2B Clone #S59-36 (MBS800075)
Streptavidin Conjugated Antibody: NR2B Clone #S59-36 (MBS800105)
Matching Pairs
Unconjugated Antibody: GluN2B/NR2B Clone #S59-36 (MBS800075)
ATTO 680 Conjugated Antibody: NR2B Clone #S59-36 (MBS800526)
Matching Pairs
Unconjugated Antibody: GluN2B/NR2B Clone #S59-36 (MBS800075)
HRP Conjugated Antibody: NR2B Clone #S59-36 (MBS800863)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q00960
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
S59-36
Host
Mouse
Species Reactivity
Human, Rat, Mouse
Specificity
Detects ~166kDa. No cross-reactivity against NR2A
Form/Format
Protein G Purified
Concentration
1mg/mL (lot specific)
Certificate of Analysis
1ug/mL was sufficient for detection of NR2B in 10ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Immunogen
Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B.
Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Cellular Localization
Cell membrane, Cell Junction, synapse, postsynaptic cell membrane
Research Areas
Neuroscience, Ion Channels, Neurotransmitter Receptors, Glutamate Receptors, NMDA Receptors, Neurodegeneration.
Conjugates
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
Preparation and Storage
Store at -20°C. 1 year+.
Avoid freeze/thaw cycles.
Shipping Temperature: 4°C
Other Notes
Small volumes of anti-Grin2b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Grin2b antibody
Background Info: Detects ~166kDa. No cross-reactivity against NR2A

Scientific Background: NR2B containing receptors have been implicated in synaptic plasticity, memory formation and pain modulation (1). Studies suggest that the NR2B subunit of glutamate receptors may be potential targets for relieving pain; NR2B may be a probable target for anti-nociceptive drugs, and may also be useful as analgesics (2).
Product Categories/Family for anti-Grin2b antibody
Applications Tested/Suitable for anti-Grin2b antibody
WB, IHC, ICC/IF, IP, AM
Application Notes for anti-Grin2b antibody
WB: (1:1000)
IHC: (1:1000)
ICC/IF (1:1000).

Optimal dilutions for assays should be determined by the user.

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-Grin2b antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything positive.
anti-Grin2b antibody Immunocytochemistry/Immunofluorescence (ICC/IF) (ICC) image
Western Blot (WB) of anti-Grin2b antibody
Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:250.
anti-Grin2b antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-Grin2b antibody
Immunohistochemistry analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining, and dermal staining.
anti-Grin2b antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-Grin2b antibody
Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
anti-Grin2b antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for Grin2b. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 2B
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, N-methyl D-aspartate 2B
NCBI Official Symbol
Grin2b  [Similar Products]
NCBI Official Synonym Symbols
GluN2B
  [Similar Products]
NCBI Protein Information
glutamate receptor ionotropic, NMDA 2B; NR2B; NMDAR2B; glutamate receptor, ionotropic, NMDA2B; N-methyl D-aspartate receptor subtype 2B; glutamate [NMDA] receptor subunit epsilon-2
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 2B
UniProt Synonym Protein Names
Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B
UniProt Gene Name
Grin2b  [Similar Products]
UniProt Synonym Gene Names
GluN2B; NMDAR2B; NR2B  [Similar Products]
UniProt Entry Name
NMDE2_RAT
NCBI Summary for Grin2b
subunit of the NMDA receptor glutamate gated ion channel; displays voltage dependent sensitivity to magnesium, calcium permeability, and glycine regulated activity; may facilitate learning and long term potentiation [RGD, Feb 2006]
UniProt Comments for Grin2b
NMDAR2B: an NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death.

Protein type: Membrane protein, multi-pass; Channel, calcium; Channel, ligand-gated; Membrane protein, integral

Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; neuron projection; cell surface; membrane; postsynaptic density; dendritic spine; terminal button; synapse; cell junction; Z disc; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: neurotransmitter binding; interleukin-1 receptor binding; zinc ion binding; calcium channel activity; beta-catenin binding; drug binding; ionotropic glutamate receptor binding; protein binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; protein heterodimerization activity; N-methyl-D-aspartate selective glutamate receptor activity; glycine binding; cell adhesion molecule binding; cation channel activity; receptor binding; D2 dopamine receptor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; fear response; behavioral fear response; startle response; response to toxin; rhythmic process; positive regulation of glutamate secretion; regulation of synaptic plasticity; response to other organism; receptor clustering; response to organic cyclic substance; positive regulation of synaptic transmission; response to carbohydrate stimulus; synaptic transmission; behavioral response to pain; response to magnesium ion; learning and/or memory; calcium ion transport; response to methylmercury; response to electrical stimulus; associative learning; cation transport; cellular response to magnesium starvation; synaptic transmission, glutamatergic; regulation of action potential; in utero embryonic development; hippocampus development; response to amphetamine; learning; response to cocaine; memory; response to manganese ion; detection of mechanical stimulus involved in sensory perception of pain; sensory organ development; regulation of postsynaptic membrane potential; response to ethanol; suckling behavior; long-term memory; response to mechanical stimulus; ionotropic glutamate receptor signaling pathway; response to cytokine stimulus; regulation of MAPKKK cascade; cerebral cortex development; response to calcium ion; regulation of excitatory postsynaptic membrane potential; multicellular organismal response to stress; response to amine stimulus
Product References and Citations for anti-Grin2b antibody
1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Zhuo M. (2009) Mol Brain. 2(1): 4 4. Gurwitz D, and Weizman A. (2002) Drug Discovery Today, 7(7): 403-406.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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