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anti-Csk antibody :: Mouse Src (active) Monoclonal Antibody

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Catalog # MBS395287
Unit / Price DISCONTINUED
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Western Blot/Blotting
Product Name

Src (active) (Csk), Monoclonal Antibody

Popular Item
Also Known As

Src (active)

Product Synonym Names
pp60src
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
450
Chromosome Location
Chromosome: 8; NC_005107.3 (62183286..62187910, complement). Location: 8q24
3D Structure
ModBase 3D Structure for P32577
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
28
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Purified from ascites by affinity chromatography.
Form/Format
Unconjugated. Provided as solution in phosphate buffered saline, pH 7.2, with 1% BSA and 0.1% sodium azide
Immunogen
KLH conjugated peptide corresponding to amino acid residues 519-536 of human c-Src.
Positive Control
Human MCF-7 cells, mouse 3T3-L1 cells, and rat PC-12 cells.
Preparation and Storage
Product should be stored at -20 degree C. Stability: Products are stable for one year from purchase when stored properly.
Other Notes
Small volumes of anti-Csk antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Csk antibody
This monoclonal antibody recognizes an active form of c-Src which is not phosphorylated at tyrosine 529. Src (also known as pp60 src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. The tyrosine 529 is located near the carboxyl terminus of Src and acts as a negative regulator, in that Src is held in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus when tyrosine 529 is phosphorylated by Csk. This conformation blocks phosphorylation of tyrosine 418 at the catalytic domain, thereby preventing Src activation. When tyrosine 529 is dephosphorylated, tyrosine 418 can be maximally phosphorylated and Src becomes active. This antibody also recognizes the other members of Src family including Fyn, Yes, and Fgr.
Product Categories/Family for anti-Csk antibody
Applications Tested/Suitable for anti-Csk antibody
Western Blot (WB), Iimmunohistochemistry (IHC), Immuncytochemistry (ICC)
Application Notes for anti-Csk antibody
WB: The recommended concentration is 1ug/ml.

Western Blot/Blotting of anti-Csk antibody
Western blot analysis using active-Src antibody, clone 28 , on MCF-7 (1), mouse 3T3-L1 (2) and rat PC-12 (3) cell lysates. Antibody used at 1 ug/ml. Detected using goat F(ab')2 anti-mouse IgG AP conjugate at 1:2000 dilution.
anti-Csk antibody Western Blot/Blotting (WB) image
NCBI/Uniprot data below describe general gene information for Csk. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
50,746 Da[Similar Products]
NCBI Official Full Name
tyrosine-protein kinase CSK
NCBI Official Synonym Full Names
c-src tyrosine kinase
NCBI Official Symbol
NCBI Protein Information
tyrosine-protein kinase CSK; C-SRC kinase; protein-tyrosine kinase (CSK)
UniProt Protein Name
Tyrosine-protein kinase CSK
UniProt Synonym Protein Names
C-Src kinase
Protein Family
UniProt Gene Name
UniProt Entry Name
CSK_RAT
UniProt Comments for Csk
Function: Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK

By similarity. Ref.3 Ref.4

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Homodimer (via SH3-domain). Interacts with PTPN8. Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1; these interactions serve to recruit CSK to the membrane where it can phosphorylate and inhibit Src-family kinases. Interacts with SRCIN1. Interacts with RHOH. Interacts (via SH2 domain) with SCIMP

By similarity. Ref.5 Ref.6

Subcellular location: Cytoplasm

By similarity. Cell membrane

By similarity. Note: Mainly cytoplasmic, also present in lipid rafts

By similarity.

Tissue specificity: Enriched in lymphoid tissues.

Domain: The architecture of this protein is similar to that of Src-family kinases (SFKs) with one N-terminal SH3 domain, one SH2 domain, and a C-terminal kinase domain

By similarity.

Post-translational modification: Phosphorylated at Ser-364 by PKA, leading to increased activity. Autophosphorylated

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.Contains 1 protein kinase domain.Contains 1 SH2 domain.Contains 1 SH3 domain.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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