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anti-CRYAA antibody :: Mouse anti-Human, Rat A crystallin A Monoclonal Antibody

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Catalog # MBS200007
Unit / Price
  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Western Blot (WB)
Product Name

A crystallin A (CRYAA), Monoclonal Antibody

Popular Item
Also Known As

A crystallin A antibody

Product Synonym Names
Heat shock protein beta-4; HspB4; Alpha-crystallin A chain; Heat shock protein beta 4; Acry-1; Alpha crystallin A chain; CRYA 1; CRYA1; CRYAA; Crystallin Alpha 1; Crystallin alpha A; HSPB 4; Zonular Central Nuclear Cataract
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
173
OMIM
U05569 mRNA
3D Structure
ModBase 3D Structure for P02489
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
c9F2
Host
Mouse
Species Reactivity
Human, Rat
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human Crystallin alpha A (1-173aa) purified from E. coli
Clone Note
Anti-human Crystallin alpha A mAb, clone c9F2, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant Crystallin alpha A.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-CRYAA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CRYAA antibody
alpha-crystallins, a major protein of the ocular lens, are thought to play a role in maintaining lens transparency, which are composed of two gene products alpha-A and alpha-B, for acidic and basic, respectively. alpha-crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP). They act as molecular chaperones and hold unfolded or misfolded proteins in large, water-soluble low molecular weight aggregates. These heterogeneous aggregates consist of 30-40 subunits of the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of alpha-crystallins are an autokinase activity and the participation in the intracellular architecture.
Product Categories/Family for anti-CRYAA antibody
Applications Tested/Suitable for anti-CRYAA antibody
ELISA (EIA), Western Blot (WB) (Cell lysate)
Application Notes for anti-CRYAA antibody
WB: 1:500 ~ 1:2000.
Recommended starting dilution : 1:1000.

Western Blot (WB) of anti-CRYAA antibody
Mouse eye extracts and recombinant proteins (Crystallin-alpha A and B) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Crystallin alpha A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. This antibody is not shown cross-activity about Crystallin alpha B. A : Crystallin-alpha A recombinant protein B : Crystallin-alpha B recombinant protein C : Mouse eye lysates The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: Crystallin alpha A Transfected 293T cell lysate
anti-CRYAA antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for CRYAA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
19,909 Da[Similar Products]
NCBI Official Full Name
alpha-crystallin A chain
NCBI Official Synonym Full Names
crystallin alpha A
NCBI Official Symbol
CRYAA  [Similar Products]
NCBI Official Synonym Symbols
CRYA1; HSPB4; CTRCT9
  [Similar Products]
NCBI Protein Information
alpha-crystallin A chain
UniProt Protein Name
Alpha-crystallin A chain
UniProt Synonym Protein Names
Heat shock protein beta-4; HspB4
Protein Family
UniProt Gene Name
CRYAA  [Similar Products]
UniProt Synonym Gene Names
CRYA1; HSPB4; HspB4  [Similar Products]
NCBI Summary for CRYAA
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq, Jan 2014]
UniProt Comments for CRYAA
Contributes to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.
Product References and Citations for anti-CRYAA antibody
Fujii N., et al., (2003) Mol. Vis. 9: 915-22; Laksanalamai P., et al., (2004) Extremophiles 8: 1-11

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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