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anti-AP2A1 antibody :: Mouse anti-Human, Rat alpha-Adaptin Monoclonal Antibody

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Catalog # MBS175087
Unit / Price
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  0.1 mg  /  $280 +1 FREE 8GB USB
anti-AP2A1 antibody
Product Name

alpha-Adaptin (AP2A1), Monoclonal Antibody

Full Product Name

Anti-alpha-Adaptin antibody (monoclonal)

Product Synonym Names
AP-2 complex subunit alpha-1; adaptor-related protein complex 2, alpha 1 subunit; ADTAA; CLAPA1; AP2-ALPHA
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
601026
3D Structure
ModBase 3D Structure for O95782
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
Ada-1
Host
Mouse
Species Reactivity
Human, Rat
Purity/Purification
Ascites
Form/Format
Lyophilized
Immunogen
AP-2 adaptor polypeptides from bovine brain.
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Reconstitution
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-AP2A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AP2A1 antibody
Description: Mouse IgG monoclonal antibody for alpha-Adaptin, adaptor-related protein complex 2, alpha 1 subunit (AP2A1) detection. Tested with WB in Human, rat. No cross reactivity with other proteins. Uniprot ID: O95782
Background: Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1(HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al.(2001) identified 3 HIP1-associated proteins, clathrin heavy chain(CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD(adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.
Applications Tested/Suitable for anti-AP2A1 antibody
Western Blot (WB)
NCBI/Uniprot data below describe general gene information for AP2A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
105,361 Da
NCBI Official Full Name
AP-2 complex subunit alpha-1
NCBI Official Synonym Full Names
adaptor-related protein complex 2, alpha 1 subunit
NCBI Official Symbol
AP2A1  [Similar Products]
NCBI Official Synonym Symbols
ADTAA; CLAPA1; AP2-ALPHA
  [Similar Products]
NCBI Protein Information
AP-2 complex subunit alpha-1; alpha1-adaptin; alpha-adaptin A; adaptin, alpha A; 100 kDa coated vesicle protein A; adaptor protein complex AP-2 subunit alpha-1; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptor-related protein complex 2 subunit alpha-1; clathrin assembly protein complex 2 alpha-A large chain; plasma membrane adaptor HA2/AP2 adaptin alpha A subunit; clathrin-associated/assembly/adaptor protein, large, alpha 1
UniProt Protein Name
AP-2 complex subunit alpha-1
UniProt Synonym Protein Names
100 kDa coated vesicle protein A; Adaptor protein complex AP-2 subunit alpha-1; Adaptor-related protein complex 2 subunit alpha-1; Alpha-adaptin A; Alpha1-adaptin; Clathrin assembly protein complex 2 alpha-A large chain; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
Protein Family
UniProt Gene Name
AP2A1  [Similar Products]
UniProt Synonym Gene Names
ADTAA; CLAPA1  [Similar Products]
UniProt Entry Name
AP2A1_HUMAN
NCBI Summary for AP2A1
This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for AP2A1
AP2A1: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C- terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. Belongs to the adaptor complexes large subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Vesicle

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: clathrin coat of trans-Golgi network vesicle; membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane; AP-2 adaptor complex; cytosol

Molecular Function: protein C-terminus binding; protein binding; protein kinase binding; protein transporter activity

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; axon guidance; synaptic transmission; intracellular protein transport; viral reproduction; nerve growth factor receptor signaling pathway; ephrin receptor signaling pathway; Golgi to endosome transport; antigen processing and presentation of exogenous peptide antigen via MHC class II; endocytosis; regulation of defense response to virus by virus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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