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anti-CD34 antibody :: Mouse CD34 Monoclonal Antibody

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Catalog # MBS438123
Unit / Price
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  0.5 mL  /  $300 +1 FREE 8GB USB
Testing Data
Product Name

CD34, Monoclonal Antibody

Popular Item
Full Product Name

CD34

Product Synonym Names
Hematopoietic Progenitor Cell Antigen; HPCA1; Mucosialin
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
142230
3D Structure
ModBase 3D Structure for P28906
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
ICO-115
Host
Mouse
Species Reactivity
Human and Rat. Others not known.
Specificity
This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.
Form/Format
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM pbs with 0.05% BSA and 0.05% azide.
Localization
Cell surface
Positive Control
KG-1 cells, Tonsil, or Angiosarcoma
Human Chromosomal Location
1q32.2
Immunogen
Blast cells of a chronic myeloid leukemia patient
Quantity and Buffer
0.5 ml at 100ug/ml; Conjugated to PE
Preparation and Storage
Store at 2 to 8°C. Antibody is stable for 24 months.
Other Notes
Small volumes of anti-CD34 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD34 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD34 antibody
Flow Cytometry 5ul (0.5ug) per test per one million cells Optimal dilution for a specific application should be determined.

Testing Data of anti-CD34 antibody
Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 Ab (ICO-115) (red) and isotype control Ab (green). PPI negative cell population was gated for analysis.
anti-CD34 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for CD34. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
90-110kDa
NCBI Official Full Name
hematopoietic progenitor cell antigen CD34 isoform a
NCBI Official Synonym Full Names
CD34 molecule
NCBI Official Symbol
NCBI Protein Information
hematopoietic progenitor cell antigen CD34; CD34 antigen
UniProt Protein Name
Hematopoietic progenitor cell antigen CD34
UniProt Synonym Protein Names
CD_antigen: CD34
UniProt Gene Name
UniProt Entry Name
CD34_HUMAN
NCBI Summary for CD34
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
UniProt Comments for CD34
CD34: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Belongs to the CD34 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: intercellular bridge; integral to plasma membrane; perinuclear region of cytoplasm; lysosome; apical plasma membrane; cytoplasm; basal plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: sulfate binding; carbohydrate binding; transcription factor binding

Biological Process: positive regulation of odontogenesis; regulation of immune response; negative regulation of nitric oxide biosynthetic process; glomerular filtration; cell motility involved in cell locomotion; negative regulation of blood coagulation; negative regulation of tumor necrosis factor production; signal transduction; positive regulation of interleukin-10 production; cell proliferation; positive regulation of angiogenesis; cell-cell adhesion; regulation of blood pressure; endothelial cell proliferation; tissue homeostasis; hemopoiesis; leukocyte migration
Product References and Citations for anti-CD34 antibody
1. Felshow DM et al. Blood 97:3768-3775 (2001).
2. Sato T et al. Blood 94:2548-2554 (1999).

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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