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anti-BBC3 antibody :: Mouse anti-Human, Rat PUMA Monoclonal Antibody

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Catalog # MBS150004
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
Western Blot (WB)
Product Name

PUMA (BBC3), Monoclonal Antibody

Full Product Name

PUMA Antibody [10D4G7]

Product Synonym Names
PUMA Antibody [10D4G7] : JFY1; PUMA; JFY-1; BCL2 binding component 3
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
605854
3D Structure
ModBase 3D Structure for Q9BXH1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10D4G7
Host
Mouse
Species Reactivity
Human, Rat
Purity/Purification
PUMA Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
Recombinant protein corresponding to amino acids 76 - 170 of human PUMA-alpha.
Buffer
PUMA Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
PUMA monoclonal antibody can be stored at -20 degree C, stable for one year.
Other Notes
Small volumes of anti-BBC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BBC3 antibody
PUMA Monoclonal Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMAα and PUMAβ. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Applications Tested/Suitable for anti-BBC3 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-BBC3 antibody
PUMA antibody can be used for detection of PUMA by Western blot at 2.5 to 5 mug/mL.

Western Blot (WB) of anti-BBC3 antibody
Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at (A) 2.5 and (B) 5 μg/mL.
anti-BBC3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for BBC3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
10,326 Da
NCBI Official Full Name
Bcl-2-binding component 3
NCBI Official Synonym Full Names
BCL2 binding component 3
NCBI Official Symbol
NCBI Official Synonym Symbols
JFY1; PUMA; JFY-1
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NCBI Protein Information
bcl-2-binding component 3; p53 up-regulated modulator of apoptosis
UniProt Protein Name
Bcl-2-binding component 3
UniProt Synonym Protein Names
JFY-1; p53 up-regulated modulator of apoptosis
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
BBC3_HUMAN
NCBI Summary for BBC3
This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
UniProt Comments for BBC3
PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; release of sequestered calcium ion into cytosol; positive regulation of neuron apoptosis; reduction of endoplasmic reticulum calcium ion concentration; determination of adult life span; response to DNA damage stimulus; negative regulation of growth
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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