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anti-CA9 antibody :: Mouse anti-Human, Rat Renal Cell Carcinoma Monoclonal Antibody

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Catalog # MBS370104
Unit / Price
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  5 Control Slides  /  $130 +1 FREE 8GB USB
  0.1 mL (Concentrate)  /  $180 +1 FREE 8GB USB
  3 ml (Prediluted)  /  $190 +1 FREE 8GB USB
  7 mL (Prediluted)  /  $245 +1 FREE 8GB USB
  0.5 mL (Concentrate)  /  $255 +1 FREE 8GB USB
  1 mL (Concentrate)  /  $410 +1 FREE 8GB USB
  15 mL (Prediluted)  /  $410 +1 FREE 8GB USB
Product Name

Renal Cell Carcinoma (CA9), Monoclonal Antibody

Popular Item
Full Product Name

Renal Cell Carcinoma

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 9; NC_000009.11 (35673915..35681156). Location: 9p13.3
OMIM
603179
3D Structure
ModBase 3D Structure for Q16790
Clonality
Monoclonal
Isotype
IgG1/K
Clone Number
PN-15
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Anti-Renal Cell Carcinoma is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Control
Kidney, Renal Cell Carcinoma
Localization
Cytoplasmic, Membranous
Reactivity Note
Paraffin, Frozen
Preparation and Storage
Store at 2°C - 8°C
Stability: 3 years
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.
Other Notes
Small volumes of anti-CA9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CA9 antibody
Renal Cell Carcinoma, also known as a Gurnistical Tumor, is the most common form of kidney cancer arising from the renal tubule. It is also the most common type of kidney cancer in adults. Initial treatment is surgery because it is notoriously resistant to radiation therapy and chemotherapy, although some cases respond to immunotherapy. Renal Cell Carcinoma antibody recognizes a 200 kDa glycoprotein localized in the brush border of the proximal renal tubule. This antibody immunoreacts with approximately 90% of Primary Renal Cell Carcinomas and approximately 85% of Metastatic Renal Cell Carcinomas. Other tumors that may react with this antibody are Parathyroid Adenoma, an occasional Breast Carcinoma. Nephroblastoma, Oncocytoma, Mesoblastic Nephroma, Transitional Cell Carcinoma, and Angiomyolipoma are not labeled with this antibody.
Application Notes for anti-CA9 antibody
Recommended IHC Protocol Pretreatment:
1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides.
2. Air dry for 2 hours at 58°C
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA.
5. Any of three heating methods may be used:
a. Electric Pressure Cooker.
Place standoff rack at base of pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b. Water Bath Method.
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a water bath set at 95°-99° C. lncubate for 30-60 minutes.
c. Conventional Steamer Method.
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes. .
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. Wash slides with IHC wash buffer or DI water.
8. Continue IHe staining protocol.!!

Dilution Information of anti-CA9 antibody
anti-CA9 antibody Dilution Information image
Immunohistochemistry (IHC) of anti-CA9 antibody
anti-CA9 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) Protocol of anti-CA9 antibody
anti-CA9 antibody Immunohistochemistry (IHC) Protocol (IHC) image
NCBI/Uniprot data below describe general gene information for CA9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
49,698 Da[Similar Products]
NCBI Official Full Name
renal cell carcinoma associated antigen G250
NCBI Official Synonym Full Names
carbonic anhydrase IX
NCBI Official Symbol
NCBI Official Synonym Symbols
MN; CAIX
  [Similar Products]
NCBI Protein Information
carbonic anhydrase 9; pMW1; CA-IX; P54/58N; membrane antigen MN; carbonic dehydratase; carbonate dehydratase IX; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250
UniProt Protein Name
Carbonic anhydrase 9
UniProt Synonym Protein Names
Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
G250; MN; CA-IX; CAIX  [Similar Products]
UniProt Entry Name
CAH9_HUMAN
NCBI Summary for CA9
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jul 2008]
UniProt Comments for CA9
Function: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Ref.12

Catalytic activity: H2CO3 = CO2 + H2O.

Cofactor: Zinc.

Enzyme regulation: Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Ref.10 Ref.11 Ref.14 Ref.15

Subunit structure: Forms oligomers linked by disulfide bonds. Ref.12 Ref.17

Subcellular location: Nucleus. Nucleus › nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection › microvillus membrane; Single-pass type I membrane protein. Note: Found on the surface microvilli and in the nucleus, particularly in nucleolus. Ref.8

Tissue specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

Induction: By hypoxia. Ref.10 Ref.11 Ref.12 Ref.14 Ref.15

Post-translational modification: Asn-346 bears high-mannose type glycan structures.

Sequence similarities: Belongs to the alpha-carbonic anhydrase family.

Biophysicochemical propertiespH dependence:Optimum pH is 6.5. Ref.17
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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