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anti-SUMO2 antibody :: Mouse SUMO-2 Monoclonal Antibody

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Catalog # MBS439221
Unit / Price
  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  7 ml (Prediluted)  /  $270 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

SUMO-2 (SUMO2), Monoclonal Antibody

Popular Item
Also Known As

SUMO-2 Mouse Monoclonal Antibody

Product Synonym Names
HSMT3; Sentrin 2; Small ubiquitin like modifier 2; Small ubiquitin like modifier protein 3; SMT3A; SMT3B; SMT3 homolog 1 (SMT3H1); SMT3 homolog 2 (SMT3H2); SMT3 homolog; SMT3 suppressor of mif two 3 homolog 1; SMT3 suppressor of mif two 3 homolog 2; SMT3 suppressor of mif two 3 homolog 3; Suppressor of mif two 3 homolog 2; Suppressor of mif two 3 homolog 3; Ubiquitin like protein SMT3A; Ubiquitin like protein SMT3B
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
141
OMIM
602231
3D Structure
ModBase 3D Structure for P55854
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
SPM621
Host
Mouse
Species Reactivity
Human, Rat.
Predicted to show a broad species reactivity
Specificity
The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, 2 and 3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include MDM2, p53, PML and RanGap1. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1 and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer s disease.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Cellular Localization
Nuclear
Immunogen
Recombinant human SUMO2 protein
Hu-Chromosome Location
17q25.1
Positive Control
HeLa cells or breast carcinoma
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-SUMO2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-SUMO2 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-SUMO2 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-SUMO2 antibody
Formalin-fixed, paraffin-embedded human Tonsil stained with SUMO-2 Monoclonal Antibody (SPM621)
anti-SUMO2 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for SUMO2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11-13kDa
NCBI Official Full Name
small ubiquitin-related modifier 3 isoform 2
NCBI Official Synonym Full Names
small ubiquitin-like modifier 3
NCBI Official Symbol
SUMO3  [Similar Products]
NCBI Official Synonym Symbols
SMT3A; Smt3B; SMT3H1; SUMO-3
  [Similar Products]
NCBI Protein Information
small ubiquitin-related modifier 3
UniProt Protein Name
Small ubiquitin-related modifier 3
UniProt Synonym Protein Names
SMT3 homolog 1
UniProt Gene Name
SUMO3  [Similar Products]
UniProt Synonym Gene Names
Smt3A  [Similar Products]
UniProt Entry Name
SUMO3_HUMAN
NCBI Summary for SUMO2
This gene encodes a member of the small ubiquitin-related modifier (SUMO) family of eukaryotic proteins. The encoded protein is covalently conjugated to other proteins via a post-translation modification known as sumoylation. Sumoylation may play a role in a wide variety of cellular processes, including nuclear transport, DNA replication and repair, mitosis, transcriptional regulation, and signal transduction. Alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq, Feb 2014]
UniProt Comments for SUMO2
SUMO3: Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. Covalently attached to a number of proteins. Interacts with USP25 (via ts SIM domain); the interaction sumoylates USP25 and inhibits its ubiquitin hydrolyzing activity. Interacts with SAE2 and UBE2I. Expressed predominantly in liver. Belongs to the ubiquitin family. SUMO subfamily.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: kinetochore; nucleoplasm; nucleus

Molecular Function: protein binding; protein tag

Biological Process: protein sumoylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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