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Mouse Anti-Human IgG (Fc) (gamma chain specific) Secondary Antibody

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Catalog # MBS674021
Unit / Price
  0.1 mg  /  $320 +1 FREE 8GB USB
Mouse Anti-Human IgG (Fc) (gamma chain specific) secondary antibody
Product Name

Mouse Anti-Human IgG (Fc) (gamma chain specific), Secondary Antibody

Popular Item
Also Known As

Mouse Anti-Human IgG (Fc)-APC

Product Synonym Names
Mouse Anti-Human IgG (Fc) - Allophycocyanin; Mouse Anti-Human IgG (Fc) (gamma chain specific)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
Mouse IgG2b kappa
Clone Number
H2
Host
Mouse
Species Reactivity
Human
Specificity
Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA; may also react with IgG from other species.
Form/Format
Allophycocyanin (APC) Conjugate
Characterization
To insure lot-to-lot consistency, each batch of product is tested by ELISA, FLISA and/or Flow Cytometry for conformance to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Other Notes
Small volumes of Mouse Anti-Human IgG (Fc) (gamma chain specific) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
ELISA; Immunocytochemistry; Immunohistochemistry (Frozen Sections); Western Blot; Indirect immunofluorescent staining; Fluorescent - Linked- Immunosorbent - assay (FLISA); Dot- and slot-immunoblotting.
Research Application(s): Indirect immunofluorescent staining of IgG+ human B lymphocytes

Immunofluorescence Suggested Dilution: <= 0.1 ug/10^6 cells

ELISA
AP conjugate 1:2,000-1:8,000
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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