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anti-POLR2A antibody :: Mouse RNA Polymerase II Monoclonal Antibody

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Catalog # MBS680017
Unit / Price
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  0.1 mg  /  $380 +1 FREE 8GB USB
anti-POLR2A antibody
Product Name

RNA Polymerase II (POLR2A), Monoclonal Antibody

Full Product Name

mAb to RNA Polymerase II

Product Synonym Names
MAb to RPB1 subunit of Yeast RNA polymerase II; RNA Pol II RPB1 Monoclonal Antibody; RNA polymerase II sub-unit 1; RPB1; CTD repeat YSPTSPS; C- terminal domain repeat YSPTSPS; DNA-directed RNA polymerase II subunit A; RNA-directed RNA polymerase II subunit RPB1; POLR2A; POLR2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
YSPTSPS repeat
Chromosome Location
Chromosome: 17; NC_000017.10 (7387698..7417935). Location: 17p13.1
OMIM
180660
3D Structure
ModBase 3D Structure for P24928
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
8WG16
Host
Mouse
Species Reactivity
Yeast, Wheat, Human, most RNAPII
Specificity
RNA Polymerase II (RNA Pol II RPB1 human and yeast)
Form/Format
Affinity Purified
Concentration
1 mg/ml in PBS pH 7.2 (lot specific)
Immunogen
Purified protein
Reported Reactivity
Wheat, mouse, C. elegans, X. laevis
Cross Reactivity Note
Wheat, yeast, human, mouse, C. elegans, X. laevis and most eukaryotic RNAPII
Polyol-responsive
Yes
Reconstitution
Add 200ul of ddH2O to the tube containing antibody and mix to dissolve
Preparation and Storage
Store at -20 degree C lyophilized, 4 degree C Reconstituted, or sub-aliquot and store at -20 degree C
Other Notes
Small volumes of anti-POLR2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-POLR2A antibody
MAb to RPB1 subunit of Yeast RNA polymerase II. RPB1 is a 220 kDA subunit of the yeast RNA polymerase II.
RPB1 is a 220kDa sub-unit of RNA Polymerase II
RPB1 is a 220 kDA subunit of the yeast RNA polymerase II.

RPB1 is the largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
Applications Tested/Suitable for anti-POLR2A antibody
Western Blot (WB), ChIP, IAC, ELISA (EIA), Transcription Inhibition, Immunoprecipitation (IP)
Application Notes for anti-POLR2A antibody
Immunofluorescence (IF): Yes
Immunoaffinity Chromatography: Yes, polyol/NaCl
Immunoprecipitation (IP): Yes
Transcription Inhibition - Initiation: Yes
Western Blot: Yes
NCBI/Uniprot data below describe general gene information for POLR2A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
217,176 Da[Similar Products]
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB1
NCBI Official Synonym Full Names
polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
NCBI Official Symbol
POLR2A  [Similar Products]
NCBI Official Synonym Symbols
RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
  [Similar Products]
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; RNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB1
UniProt Synonym Protein Names
DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1
UniProt Gene Name
POLR2A  [Similar Products]
UniProt Synonym Gene Names
POLR2; RNA polymerase II subunit B1  [Similar Products]
UniProt Entry Name
RPB1_HUMAN
NCBI Summary for POLR2A
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]
UniProt Comments for POLR2A
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome. Ref.6 Ref.23

Catalytic activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. The phosphorylated C-terminal domain interacts with FNBP3 and SYNCRIP. Interacts with SAFB/SAFB1. Interacts with CCNL1 and MYO1C

By similarity. Interacts with CCNL2 and SFRS19. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with PAF1. Interacts (via C-terminus) with FTSJD2, CTDSP1 and SCAF8. Interacts via the phosphorylated C-terminal domain with WDR82 and with SETD1A and SETD1B only in the presence of WDR82. Interacts with ATF7IP. When phosphorylated at 'Ser-5', interacts with MEN1; the unphosphorylated form, or phosphorylated at 'Ser-2' does not interact. Interacts with DDX5. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.20 Ref.21 Ref.22 Ref.24 Ref.26

Subcellular location: Nucleus Ref.6.

Post-translational modification: The tandem 7 residues repeats in the C-terminal domain (CTD) can be highly phosphorylated. The phosphorylation activates Pol II. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapeptide repeat and is mediated, at least, by CDK7 and CDK9. CDK7 phosphorylation of POLR2A associated with DNA promotes transcription initiation by triggering dissociation from DNA. Phosphorylation also takes place at 'Ser-7' of the heptapepdtide repeat, which is required for efficient transcription of snRNA genes and processing of the transcripts. The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphatases, and a 'CTD code' that specifies the position of Pol II within the transcription cycle has been proposed. Ref.27 Ref.28 Ref.29 Ref.33 Ref.36 Ref.38Dephosphorylated by the protein phosphatase CTDSP1. Ref.27 Ref.28 Ref.29 Ref.33 Ref.36 Ref.38Ubiquitinated by WWP2 leading to proteasomal degradation

By similarity. Following UV treatment, the elongating form of RNA polymerase II (RNA pol IIo) is ubiquitinated UV damage sites without leading to degradation: ubiquitination is facilitated by KIAA1530/UVSSA and promotes RNA pol IIo backtracking to allow access to the nucleotide excision repair machinery. Ref.37Methylated at Arg-1810 by CARM1. Methylation occurs only when the CTD is hypophosphorylated, and phosphorylation at Ser-1805 and Ser-1808 prevent methylation (in vitro). It is assumed that methylation occurs prior to phosphorylation and transcription initiation. CTD methylation may facilitate the expression of select RNAs. Ref.27 Ref.28 Ref.29 Ref.33 Ref.36 Ref.38

Miscellaneous: The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion temporarily coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits. The ribonucleoside triphosphate is transferred by a rotation to the nucleotide addition (A) site for pairing with the template DNA. The catalytic A site involves three conserved aspartate residues of the RNA Pol II largest subunit which permanently coordinate a second magnesium ion.

Sequence similarities: Belongs to the RNA polymerase beta' chain family.
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Disclaimer
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