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Mouse EF1, alpha Monoclonal Antibody

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Catalog # MBS601793
Unit / Price
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  0.2 mg  /  $655 +1 FREE 8GB USB
anti-EF1, alpha antibody
Product Name

EF1, alpha, Monoclonal Antibody

Full Product Name

EF1, alpha (Elongation Factor 1a)

Product Synonym Names
Anti -EF1, alpha (Elongation Factor 1a)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
0.T.35
Host
Mouse
Species Reactivity
Mammal, Yeast
Specificity
Recognizes EF-1a (Elongation factor-1a) at 53kD. 38kD protein occasionally detected in A431 cell lysate. Species Crossreactivity: Trypanosomes, yeast and mammals.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Immunogen
Crude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-EF1, alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-EF1, alpha antibody
Human eukaryotic translation elongation factor 1 alpha has two isoforms, alpha 1 and alpha 2. The alpha 1 isoform gene encodes a subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. The alpha 1 isoform is expressed in brain, placenta, lung, liver, kidney, and pancreas. The other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. The alpha 1 isoform is identified as an autoantigen in 66% of patients with Felty syndrome. The gene for the alpha 1 isoform has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. The gene for the alpha 2 may be critical in the development of ovarian cancer.
Applications Tested/Suitable for anti-EF1, alpha antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-EF1, alpha antibody
Suitable for use in Western Blot and Immunoprecipiation.
Dilution: Western Blot: 0.5-2ug/ml detects EF-1a in RIPA lysates from non-stimulated A431 cells. Cell lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with E2212 (0.5ug/ml). Proteins were visualized using an IgG (HRP) Gt xMo and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitated EF-1 a from an A431 RIPA cell lysate.
Optimal dilution determined by the researcher.
NCBI/Uniprot data below describe general gene information for EF1, alpha. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
NCBI Official Full Name
EF1, partial
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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