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anti-CD20 antibody :: Mouse CD20 Monoclonal Antibody

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Catalog # MBS212274
Unit / Price
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  0.2 mg  /  $590 +1 FREE 8GB USB
Product Name

CD20, Monoclonal Antibody

Popular Item
Full Product Name

MOUSE ANTI HUMAN CD20

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
112210
3D Structure
ModBase 3D Structure for P11836
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
2H7
Host
Mouse
Species Reactivity
Rhesus Monkey
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-CD20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD20 antibody
Mouse anti Human CD20 antibody, clone 2H7 recognizes the human CD20 cell surface antigen, a 33-37 kDa non-glycosylated phosphoprotein. The CD20 antigen is expressed during pre-B-cell development. It is present on both resting and activated B-cells but is lost prior to terminal B-cell differentiation into plasma cells. The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that Mouse anti Human CD20 antibody, clone 2H7 only recognizes human CD20 in its native oligomeric form (Polyak et al. 2002).
Applications Tested/Suitable for anti-CD20 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS)
Application Notes for anti-CD20 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100

Testing Data of anti-CD20 antibody
Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:FITC (MBS210754)
anti-CD20 antibody Testing Data image
Testing Data of anti-CD20 antibody
Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:Alexa Fluor 647 (MBS212274A647)
anti-CD20 antibody Testing Data image
Testing Data of anti-CD20 antibody
Staining of human peripheral blood lymphocytes with Mouse anti Human CD20
anti-CD20 antibody Testing Data image
Testing Data of anti-CD20 antibody
Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:Biotin (MBS210355)
anti-CD20 antibody Testing Data image
Testing Data of anti-CD20 antibody
Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:Alexa Fluor 488 (MBS212274A488)
anti-CD20 antibody Testing Data image
Testing Data of anti-CD20 antibody
Staining of human peripheral blood lymphocytes with Mouse anti Human CD20: Pacific Blue (MBS212274PB)
anti-CD20 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for CD20. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
14,581 Da
NCBI Official Full Name
B-lymphocyte antigen CD20
NCBI Official Synonym Full Names
membrane-spanning 4-domains, subfamily A, member 1
NCBI Official Symbol
MS4A1  [Similar Products]
NCBI Official Synonym Symbols
B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
  [Similar Products]
NCBI Protein Information
B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16
UniProt Protein Name
B-lymphocyte antigen CD20
UniProt Synonym Protein Names
B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD_antigen: CD20
Protein Family
UniProt Gene Name
MS4A1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
CD20_HUMAN
NCBI Summary for CD20
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
UniProt Comments for CD20
MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family.

Protein type: Membrane protein, integral; Cell surface; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane

Molecular Function: epidermal growth factor receptor binding

Biological Process: B cell proliferation; humoral immune response

Disease: Immunodeficiency, Common Variable, 5
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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