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anti-ADIPOQ antibody :: Mouse anti-Mouse ADIPONECTIN Monoclonal Antibody

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Catalog # MBS214456
Unit / Price
  0.1 mg  /  $465 +1 FREE 8GB USB
Product Name

ADIPONECTIN (ADIPOQ), Monoclonal Antibody

Also Known As

MOUSE ANTI HUMAN ADIPONECTIN

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
244
OMIM
125853
3D Structure
ModBase 3D Structure for Q15848
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5H7
Host
Mouse
Species Reactivity
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Perservative Stabilisers
0.1% Sodium Azide (NaN3)
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-ADIPOQ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ADIPOQ antibody
Mouse anti Human adiponectin, clone 5H7 recognizes the collagen-like domain of human adiponectin, also known as Acrp30, Gelatin-binding protein, Adipose most abundant gene transcript 1 protein, Adipocyte complement-related 30 kDa protein or apM-1. Adiponectin a 244 amino acid ~30kDa major adipokine secreted into the bloodstream from adipose tissue into the circulation where it exists as three distinct, low, medium and high molecular weight oligomeric forms (UniProt: Q15848). Circulating adiponectin levels are correlated with insulin sensitivity and decreased levels parallel the change in insulin sensitivity during the progression to type II diabetes (Heilbronn et al. 2011). Mutations in the adiponectin gene can also lead to adiponectin deficiency charactrized by obesity, diabetes, high blood pressure and circulating cholerterol (Takahashi et al. 2000). Mouse anti Human adiponectin, clone 5H7 had been used succesfully as a detection reagent in the development of sensitive sandwich ELISA's in conjunction with Mouse anti adiponectin clones Adn27 or Adn36 as capture reagents.
Applications Tested/Suitable for anti-ADIPOQ antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-ADIPOQ antibody
Western Blot: This item detects a band of approximately 64kDa in mouse liver cell lysates.
Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/2000

Testing Data of anti-ADIPOQ antibody
Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin (MBS219142) as a capture reagent and biotinylated Mouse anti Human adiponectin as a detection reagent with recombinant human adiponectin (MBS232423) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . A plasma sample diluted 1:4 (green) is shown
anti-ADIPOQ antibody Testing Data image
Testing Data of anti-ADIPOQ antibody
Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin (MBS219143) as a capture reagent and biotinylated Mouse anti Human adiponectin as a detection reagent with recombinant human adiponectin (MBS232423) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . A plasma sample diluted 1:4 (green) is shown
anti-ADIPOQ antibody Testing Data image
NCBI/Uniprot data below describe general gene information for ADIPOQ. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
26,414 Da[Similar Products]
NCBI Official Full Name
adiponectin
NCBI Official Synonym Full Names
adiponectin, C1Q and collagen domain containing
NCBI Official Symbol
ADIPOQ  [Similar Products]
NCBI Official Synonym Symbols
ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
  [Similar Products]
NCBI Protein Information
adiponectin; 30 kDa adipocyte complement-related protein; adipocyte complement-related 30 kDa protein; adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; gelatin-binding protein 28
UniProt Protein Name
Adiponectin
UniProt Synonym Protein Names
30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein
Protein Family
UniProt Gene Name
ADIPOQ  [Similar Products]
UniProt Synonym Gene Names
ACDC; ACRP30; APM1; GBP28; ACRP30; apM-1  [Similar Products]
UniProt Entry Name
ADIPO_HUMAN
NCBI Summary for ADIPOQ
This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]
UniProt Comments for ADIPOQ
adiponectin: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9A via the C1q domain (heterotrimeric complex). Synthesized exclusively by adipocytes and secreted into plasma.

Protein type: Endoplasmic reticulum; Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space; collagen; cell surface; endoplasmic reticulum; extracellular region

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; hormone activity; cytokine activity; sialic acid binding; receptor binding

Biological Process: circadian rhythm; negative regulation of MAP kinase activity; negative regulation of phagocytosis; negative regulation of smooth muscle cell proliferation; negative regulation of hormone secretion; response to glucocorticoid stimulus; membrane hyperpolarization; positive regulation of cellular protein metabolic process; negative regulation of smooth muscle cell migration; glucose homeostasis; negative regulation of granulocyte differentiation; positive regulation of interleukin-8 production; positive regulation of glucose import; negative regulation of gluconeogenesis; response to glucose stimulus; adiponectin-mediated signaling pathway; negative regulation of protein amino acid autophosphorylation; negative regulation of blood pressure; negative regulation of cell migration; response to nutrient; protein homooligomerization; generation of precursor metabolites and energy; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of heterotypic cell-cell adhesion; positive regulation of signal transduction; glucose metabolic process; negative regulation of tumor necrosis factor production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of fat cell differentiation; negative regulation of synaptic transmission; response to sucrose stimulus; membrane depolarization; positive regulation of peptidyl-tyrosine phosphorylation; fatty acid beta-oxidation; positive regulation of fatty acid metabolic process; response to ethanol; cellular response to insulin stimulus; negative regulation of macrophage differentiation; negative regulation of low-density lipoprotein receptor biosynthetic process; negative regulation of inflammatory response; brown fat cell differentiation; response to hypoxia; fatty acid oxidation; response to activity; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; positive regulation of myeloid cell apoptosis; positive regulation of blood pressure

Disease: Adiponectin, Serum Level Of, Quantitative Trait Locus 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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