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anti-HSPA5 antibody :: Rat anti-Mouse BiP Monoclonal Antibody

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Catalog # MBS673495
Unit / Price
  0.5 mg  /  $325 +1 FREE 8GB USB
anti-HSPA5 antibody
Product Name

BiP (HSPA5), Monoclonal Antibody

Also Known As

Rat Anti-Mouse BiP-UNLB

Product Synonym Names
Rat Anti-Mouse BiP - Purified, Unlabeled Antibody; Rat Anti-Mouse BiP
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
653
Chromosome Location
Chromosome: 9; NC_000009.11 (127997127..128003661, complement). Location: 9q33.3
OMIM
138120
3D Structure
ModBase 3D Structure for P11021
Clonality
Monoclonal
Isotype
Rat IgG1 kappa
Clone Number
76-E6
Host
Rat
Species Reactivity
Mouse
Specificity
BiP (Immunoglobulin heavy chain binding protein, Mr 78 kDa)
The immunoglobulin heavy chain binding protein BiP (Binding Protein, [1]) is a member of the hsp70 family of heat shock proteins, and is identical to the glucose regulated protein grp78.2 While BiP was originally described for its function in B cells, it is now known to be distributed in a variety of tissues, if not ubiquitous. The highly conserved hsp 70 proteins have an essential physiological role in stress responses and as "molecular chaperones," which are responsible for a variety of functions such as protein transport, prevention of protein toxicity and direction of protein folding.1-5 With regard to its immunological role, BiP is a component of the endoplasmic reticulum and binds free intracellular heavy chains in nonsecreting pre-B cell lines (u+,L-) or incompletely assembled Ig precursors in H+L+ secreting hybridomas and myelomas. In the absence of light chain synthesis, heavy chains remain associated with BiP and are not secreted. BiP is an ATP binding protein and the dissociation of the BiP-heavy chain complex is probably driven by the ATPase activity attributed to BiP.7 Monoclonal antibody 76-E6 recognizes a conserved epitope localized within the region of amino acids 497 to 581 of BiP.8
Form/Format
Purified (UNLB) Antibody
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform with characteristics of a standard reference reagent using Western blotting. Representative data are included in this data sheet.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Other Notes
Small volumes of anti-HSPA5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-HSPA5 antibody
ELISA; Western Blot
Application Notes for anti-HSPA5 antibody
Research Application(s): Studies of immunoglobulin assembly and secretion
NCBI/Uniprot data below describe general gene information for HSPA5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
72,333 Da[Similar Products]
NCBI Official Full Name
BiP
NCBI Official Synonym Full Names
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
NCBI Official Symbol
HSPA5  [Similar Products]
NCBI Official Synonym Symbols
BIP; MIF2; GRP78; FLJ26106
  [Similar Products]
NCBI Protein Information
78 kDa glucose-regulated protein; OTTHUMP00000022124; immunoglobulin heavy chain-binding protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
UniProt Protein Name
78 kDa glucose-regulated protein
UniProt Synonym Protein Names
Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein
Protein Family
UniProt Gene Name
HSPA5  [Similar Products]
UniProt Synonym Gene Names
GRP78  [Similar Products]
UniProt Entry Name
GRP78_HUMAN
NCBI Summary for HSPA5
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.
UniProt Comments for HSPA5
Function: Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Ref.12

Subunit structure: Interacts with DNAJC1 (via J domain)

By similarity. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with TMEM132A and TRIM21. May form a complex with ERLEC1, OS9, SEL1L and SYVN1. Ref.16 Ref.20 Ref.21

Subcellular location: Endoplasmic reticulum lumen. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.17 Ref.18

Involvement in disease: Note=Autoantigen in rheumatoid arthritis. Ref.10

Sequence similarities: Belongs to the heat shock protein 70 family.
Product References and Citations for anti-HSPA5 antibody
1. Bole, D.G., L.M. Hendershot, and J.F. Kearney. 1986. J. Cell Biol. 102:1558.
2. Haas, I.G. 1991. Current Topics Micro. & Immunol. 167:71.
3. Hendrick, J.P. and Hartl, F.U. 1991. FASEB J. 9(15):1559.
4. Pierce, S.K. 1994. Experientia 50(11-12):1026
5. Haas, I.G. 1994. Experientia 50(11-12):1012.
6. Wu, Q. 1996.
7. Kassenbrock, C.K. and Kelly, R.B. 1989. EMBO J. 8: 233.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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