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Rat anti-Mouse BP-1 (Ly-51, 6C3) Monoclonal Antibody

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Catalog # MBS673491
Unit / Price
  0.5 mg  /  $365 +1 FREE 8GB USB
anti-BP-1 (Ly-51, 6C3) antibody
Product Name

BP-1 (Ly-51, 6C3), Monoclonal Antibody

Also Known As

Rat Anti-Mouse BP-1/Ly-51-FITC

Product Synonym Names
Rat Anti-Mouse BP-1/Ly-51 - FITC; Rat Anti-Mouse BP-1 (Ly-51,6C3)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
Rat IgG1 kappa
Clone Number
FG35.4
Host
Rat
Species Reactivity
Mouse
Specificity
BP-1/Ly-51/6C3 antigen, (aminopeptidase A, E.C.3.4.11.7, glutamylaminopeptidase)
BP-1, also known as Ly-51/6C3 antigen, is a type II homodimeric transmembrane glycoprotein formed by two disulfide-linked chains of 140 kDa.1, 2 Originally identified on the surface of pre-B cell lymphomas1, 3 the cDNA encoding BP-1/Ly-51/6C3 has been cloned and found to represent aminopeptidase A.4, 5 It is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoiesis and on a wide variety of mouse tissues known to possess aminopeptidase activity.6-8 Subsets of normal bone marrow pre-B and B cells express low levels of BP-1/Ly-51/6C3, which is rapidly upregulated on pre-B cells in the presence of IL- 7.9, 10 The BP-1/6C3 marker is commonly used to identify B cells in different stages of development.11
Form/Format
Fluorescein (FITC) Conjugate
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative flow data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Other Notes
Small volumes of anti-BP-1 (Ly-51, 6C3) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-BP-1 (Ly-51, 6C3) antibody
Flow Cytometry; Immunoprecipitation
Application Notes for anti-BP-1 (Ly-51, 6C3) antibody
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Product References and Citations for anti-BP-1 (Ly-51, 6C3) antibody
1. Cooper, M. D., D. Mulvaney, A. Coutinho, and P. A. Cazenave. 1986. Nature 321:616.
2. Wu, Q., G. F. Tidmarsh, P. A. Welch, J. H. Pierce, I. L. Weissman, and M. D. Cooper. 1989. J. Immunol. 143:3303.
3. Pillemer, E., C. Whitelock, and I. L. Weissman. 1984. Proc. Natl. Acad. Sci. USA. 81:4434.
4. Wu, Q., J. M. Lahti, G. M. Air, P. D. Burrows, and M. D. Cooper. 1990. Proc. Natl. Acad. Sci. USA. 87:993.
5. Wu, Q., L. Li, M. D. Cooper, M. Pierres, and J. P. Gorvel. 1991. Proc. Natl. Acad. Sci. U. S. A. 88:676.
6. Whitlock, C. A., G. F. Tidmarsh, C. Muller-Sieburg, and I. L. Weissman. 1987. Cell 48:1009.
7. Adkins, B., G. F. Tidmarsh, and I. L. Weissman. 1988. Immunogenetics 27:180.
8. Li, L., Q. Wu, J. Wang, R. P. Bucy, and M. D. Cooper. 1993. Tissue Antigens. 42:488.
9. Sherwood, P. J. and I. L. Weissman. 1990. Int. Immunol. 2:399.
10. Welch, P. A., P. D. Burrows, A. Namen, S. Gillis, and M. D. Cooper. 1996. Int. Immunol. 2:697.
11. Hardy, R. R., C. E. Carmack, S. A. Shinton, J. D. Kemp, and K. Hayakawa. 1991. J. Exp. Med. 173:1213.
12. Shu, F. and J. F. Kearney. 1996. Personal communication.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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