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anti-CD25 antibody :: Rat anti-Mouse CD25 Monoclonal Antibody

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Catalog # MBS520081
Unit / Price
  0.25 mg  /  $260 +1 FREE 8GB USB
Testing Data
Product Name

CD25, Monoclonal Antibody

Also Known As

Anti-Mouse CD25, Purified (Clone PC61.5.3) (rat IgG1)

Product Synonym Names
CD25, Purified (Clone PC61.5.3) (rat IgG1); Purified Anti-Mouse CD25 (IL-2R) Monoclonal Antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
160
3D Structure
ModBase 3D Structure for P25094
Clonality
Monoclonal
Isotype
Rat IgG1
Clone Number
PC61.5.3
Host
Rat
Species Reactivity
Mouse
Specificity
CD25 (IL-2Ra, p55, Ly 43) (Mouse CD25 (IL-2 receptor, a chain))
Form/Format
Purified (Purified Ig buffered in PBS and 0.02% NaN3. (Purified from bioreactor material via Protein G Chromatography))
Concentration
Antibody Concentration: 1.0 mg/ml (lot specific)
Immunogen
B6.1 CTL Cell line
Donor
OFA rat spleen
Fusion Partner
mouse myeloma line P3X63Ag8.653
Presentation
250 ug purified Ig buffered in PBS and 0.02% NaN3
Preparation and Storage
Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-CD25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD25 antibody
anti-mouse CD25 (IL-2R) monoclonal antibody reacts with the low affinity alpha chain of the interleukin-2 receptor antigen present on activated T and B cells in mice. MBS520081 inhibits IL-2 binding and IL-2 dependent proliferation. Applications include flow cytometry and immunoprecipitation. (1,2,3). This clone has also been referenced to work in immunohistochemistry (4).
Applications Tested/Suitable for anti-CD25 antibody
Immunohistochemistry (IHC), Flow Cytometry (FC), Immunoprecipitation (IP), Functional Assay (FA)

Testing Data of anti-CD25 antibody
anti-CD25 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for CD25. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
17,946 Da
NCBI Official Full Name
CD25
UniProt Protein Name
Peripheral myelin protein 22
UniProt Synonym Protein Names
Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein
UniProt Gene Name
Pmp22  [Similar Products]
UniProt Synonym Gene Names
Cd25; Pmp-22; PMP-22; SAG  [Similar Products]
UniProt Entry Name
PMP22_RAT
UniProt Comments for CD25
Function: Might be involved in growth regulation, and in myelinization in the peripheral nervous system.

Subcellular location: Cell membrane; Multi-pass membrane protein

By similarity.

Tissue specificity: Found exclusively in the peripheral nervous system. Present in both myelinating and nonmyelinating Schwann cells. Found in the tumors of Schwann cell lineage where axons are present (neurofibromas) but not where axons are absent (schwannomas). Ref.4

Developmental stage: Levels increase between embryonic day 21 (E21) and postnatal day 0 (PND0) and then gradually increase up to PND15. There is a slight increase between PND15 and adulthood. Ref.4

Induction: Strongly down-regulated in the initial phase after sciatic nerve injury.

Sequence similarities: Belongs to the PMP-22/EMP/MP20 family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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