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anti-TNFRSF8 antibody :: anti-Mouse CD30 Monoclonal Antibody

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Catalog # MBS673313
Unit / Price
  0.5 mg  /  $260 +1 FREE 8GB USB
anti-TNFRSF8 antibody
Product Name

CD30 (TNFRSF8), Monoclonal Antibody

Also Known As

Hamster Anti-Mouse CD30-UNLB

Product Synonym Names
Hamster Anti-Mouse CD30 - Purified, Unlabeled Antibody; Hamster Anti-Mouse CD30
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
595
Chromosome Location
Chromosome: 1; NC_000001.10 (12123434..12204264). Location: 1p36
OMIM
153243
3D Structure
ModBase 3D Structure for P28908
Clonality
Monoclonal
Isotype
Armenian Hamster IgG kappa
Clone Number
2SH12-5F (also known as mCD30.1)
Host
Armenian Hamster
Species Reactivity
Mouse
Specificity
Murine CD30, Mr 52 kDa (core peptide)
CD30 is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily of proteins.^1,2 Murine CD30 is expressed predominantly in the thymus, and it is inducible in mouse splenocytes stimulated with pokeweed mitogen or concanavalin A.^1 In anti-CD3 epsilon -activated spleen cells, CD30 is expressed primarily on the surface of CD8^+ T cells with peak expression on days 4 and 5. Stimulation of CD30^+ CTL lines with plate-bound anti-CD30 directly signals IL-5 but not IFN- gamma production. ^1 While these studies demonstrate that CD30 directs cytokine secretion and suggest that CD30 may play a pivotal role in the pattern of cytokine production by T cells, the precise roles of CD30 and its ligand (CD153) in T-cell development have not been clearly defined.
Form/Format
Purified (UNLB) Antibody
Immunogen
Recombinant mouse CD30 extracellular domain-mouse IgG1 fusion protein
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Other Notes
Small volumes of anti-TNFRSF8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TNFRSF8 antibody
Flow Cytometry
Application Notes for anti-TNFRSF8 antibody
Research Application(s): In vitro functional studies

Flow Cytometry Suggested Dilution: <= 3 ug/10^6 cells
NCBI/Uniprot data below describe general gene information for TNFRSF8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
63,747 Da[Similar Products]
NCBI Official Full Name
CD30 antigen
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 8
NCBI Official Symbol
TNFRSF8  [Similar Products]
NCBI Official Synonym Symbols
CD30; Ki-1; D1S166E
  [Similar Products]
NCBI Protein Information
tumor necrosis factor receptor superfamily member 8; CD30 antigen; Ki-1 antigen; CD30L receptor; OTTHUMP00000001783; OTTHUMP00000221481; cytokine receptor CD30; lymphocyte activation antigen CD30
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 8
UniProt Synonym Protein Names
CD30L receptor; Ki-1 antigen; Lymphocyte activation antigen CD30
Protein Family
UniProt Gene Name
TNFRSF8  [Similar Products]
UniProt Synonym Gene Names
CD30; D1S166E  [Similar Products]
UniProt Entry Name
TNR8_HUMAN
NCBI Summary for TNFRSF8
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]
UniProt Comments for TNFRSF8
Function: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.

Subunit structure: Interacts with TRAF1, TRAF2, TRAF3 and TRAF5. Ref.8 Ref.9 Ref.10 Ref.11

Subcellular location: Isoform Long: Cell membrane; Single-pass type I membrane protein. Isoform Short: Cytoplasm.

Post-translational modification: Phosphorylated on serine and tyrosine residues. Ref.12

Miscellaneous: Most specific Hodgkin disease associated antigen.

Sequence similarities: Contains 6 TNFR-Cys repeats.
Product References and Citations for anti-TNFRSF8 antibody
1. Bowen, M.A., R.K. Lee, G. Miragliotta, S.Y. Nam, and E.R. Podack. 1996. J. Immunol. 156:442.
2. Falini, B., et al. 1995. Blood 85:1.
3. Shanebeck, K. D., et al. 1995. Eur. J. Immunol. 25:2147.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withanti-TNFRSF8 antibody
 Reference Product  PubMed Publications
 TNFSF8 antibody  >1 publications with TNFRSF8 and TNFSF8
Organs/Tissues associated with anti-TNFRSF8 antibody
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