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Rat anti-Mouse CD31/PECAM-1 Monoclonal Antibody

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Catalog # MBS673323
Unit / Price
  0.2 mg  /  $310 +1 FREE 8GB USB
anti-CD31/PECAM-1 antibody
Product Name

CD31/PECAM-1, Monoclonal Antibody

Popular Item
Also Known As

Rat Anti-Mouse CD31/PECAM-1-PE

Product Synonym Names
Rat Anti-Mouse CD31/PECAM-1 - Phycoerythrin-Labeled; Rat Anti-Mouse CD31/PECAM-1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
Rat (Lewis) IgG2a kappa
Clone Number
390
Host
Rat
Species Reactivity
Mouse
Specificity
Mouse CD31/PECAM-1, Mr 130-140 kDa
CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and paltelets.^1-3 CD31 interacts homotypically in cell adhesion assays. MAb 390 inhibits the aggregation of L cells transfected with a variant form of CD31.^1, 3
Form/Format
R - phycoerythrin (R - PE) Conjugate
Immunogen
Mouse leukocyte cell line 32D
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform with characteristics of a standard reference reagent. Representative data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
The R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Other Notes
Small volumes of anti-CD31/PECAM-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD31/PECAM-1 antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
Application Notes for anti-CD31/PECAM-1 antibody
Research Application(s): Blocking of Cell Adhesion

Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Product References and Citations for anti-CD31/PECAM-1 antibody
1. Abelda, S.M. Personal communication
2. Buck, C.A., H.S. Baldwin, H. DeLisser, C. Mickanin, H.-M. Shen, G. Kennedy, A. Chen, J.M. Edelman, and S.M. Abelda. 1993. Comptes Rendus de l-Academie des Sciences Serie III-Sciences de la Vie-Life Sciences 316:838.
3. DeLisser, H.M., P.J. newman, and S.M. Abelda. 1993. Curr. Topics Microbiol. Immunol. 184:37.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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