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anti-CD40 antibody :: Rat anti-Mouse CD40 Monoclonal Antibody

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Catalog # MBS520120
Unit / Price
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  0.1 mg  /  $200 +1 FREE 8GB USB
  0.3 mg  /  $370 +1 FREE 8GB USB
Testing Data
Product Name

CD40, Monoclonal Antibody

Full Product Name

Anti-Mouse CD40, Biotin (Clone 3/23) (rat IgG2a)

Product Synonym Names
CD40, Biotin (Clone 3/23) (rat IgG2a); Biotin Anti-Mouse CD40 Monoclonal Antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 20; NC_000020.10 (44746899..44758384). Location: 20q12-q13.2
OMIM
109535
3D Structure
ModBase 3D Structure for P25942
Clonality
Monoclonal
Isotype
Rat IgG2a
Clone Number
2/23
Host
Rat
Species Reactivity
Mouse
Specificity
CD40 (gp39 receptor) (Mouse CD40)
Form/Format
Biotin
Concentration
Antibody Concentration: 0.1 mg/ml (lot specific)
Presentation
100 ug or 300 ug Biotin conjugated Ig buffered in PBS, 0.1% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Preparation and Storage
Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles. Check label for expiry date.
Other Notes
Small volumes of anti-CD40 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD40 antibody
anti-mouse monoclonal antibody reacts with CD40 which is expressed on B-lymphocytes and on a subset of both CD4+ and CD8+ T cells in the adult (but not neonatal) spleen1. The level of CD40 on B cells is enhanced following activation buy LPS1. The interactions of CD40 with its ligand CD40L are responsible for generating humoral responses, germinal center formation, and generating B cell memory2. Stimulating cells with immobilized 3/23 mAb increases expression of CD86 (B7.2) on B cells1,3. Though the 3/23 mAb itself is a weak mitogen, it produces a synergistic proliferative B cell response with IL-4 or with mitogenic antibodies against surface Ig 1,3. Costimulation with 3/23 mAb and IL-4 rescues B cells from apoptosis induced by hypercross-linking of sIgM or sIgD3. This antibody is suitable for use in flow cytometry. Other applications of this antibody include IHC of acetonefixed frozen sections2.
Applications Tested/Suitable for anti-CD40 antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Testing Data of anti-CD40 antibody
anti-CD40 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for CD40. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
30,619 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 5 isoform 2
NCBI Official Synonym Full Names
CD40 molecule, TNF receptor superfamily member 5
NCBI Official Symbol
NCBI Official Synonym Symbols
p50; Bp50; CDW40; TNFRSF5
  [Similar Products]
NCBI Protein Information
tumor necrosis factor receptor superfamily member 5; CD40L receptor; CD40 type II isoform; B cell-associated molecule; B cell surface antigen CD40; B-cell surface antigen CD40; CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily, member 5; nerve growth factor receptor-related B-lymphocyte activation molecule
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 5
UniProt Synonym Protein Names
B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
TNFRSF5  [Similar Products]
UniProt Entry Name
TNR5_HUMAN
NCBI Summary for CD40
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Comments for CD40
Function: Receptor for TNFSF5/CD40LG.

Subunit structure: Monomer and homodimer. The variant form found in the bladder carcinoma cell line Hu549 does not form homodimers. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Ref.11 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18

Subcellular location: Isoform I: Cell membrane; Single-pass type I membrane protein. Isoform II: Secreted.

Tissue specificity: B-cells and in primary carcinomas.

Involvement in disease: Immunodeficiency with hyper-IgM 3 (HIGM3) [MIM:606843]: A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE. It results in a profound susceptibility to bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.23

Sequence similarities: Contains 4 TNFR-Cys repeats.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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