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anti-Kl antibody :: Rat anti-Mouse Klotho Monoclonal Antibody

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Catalog # MBS690455
Unit / Price
  0.1 mg  /  $490 +1 FREE 8GB USB
anti-Kl antibody
Product Name

Klotho, Monoclonal Antibody

Popular Item
Also Known As

Rat Anti-Mouse Klotho

Product Synonym Names
Anti-mouse Klotho (#4F54)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1014
Chromosome Location
Chromosome: 5; NC_000071.6 (150952607..150993817). Location: 5; 5 G3
3D Structure
ModBase 3D Structure for O35082
Clonality
Monoclonal
Isotype
IgG2
Clone Number
(4F54)
Host
Rat
Species Reactivity
Mouse
Specificity
This antibody detects mouse Klotho in Western blotting.
Purity/Purification
Protein A/G chromatography
Form/Format
The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at
Antibody Generation
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant protein of Klotho. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
Antigen
Recombinant mouse Klotho
Reconstitution buffer
PBS (sterile)
Reconstitution
Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.
Preparation and Storage
Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-Kl antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Kl antibody
The Klotho proteins, Klotho and Klotho beta (beta KL), are type I transmembrane glycoproteins with extracellular regions that contain two beta-glycosidase-like repeats. Klotho has both membrane-bound and soluble forms; the soluble form predominates and consists of only the first beta-glycosidase-like repeat. Klotho has beta-glucuronidase activity against select steroid beta-glucuronides, appears to regulate calcium and vitamin D levels in serum, and is associated with premature aging. Klotho beta, a divergent structural member of the glycosidase I superfamily, is primarily expressed in the liver and pancreas, with lower expression in adipose tissue. Klotho beta mediates the interaction of FGF-15 (human FGF-19) with FGF Receptor 4 in the liver, which negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes. Klotho beta is also a cofactor for the interaction of FGF-21 with FGF Receptor 1c in adipocytes. The expression pattern of Klotho beta suggests it may play a role in lipid metabolism.
Applications Tested/Suitable for anti-Kl antibody
Immunohistochemistry (IHC) - Formalin, Western Blot (WB)
Application Notes for anti-Kl antibody
Western blot: 1:500-1000
IHC (Frozen): 1:50-200
NCBI/Uniprot data below describe general gene information for Kl. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
klotho
NCBI Official Synonym Full Names
klotho
NCBI Official Symbol
NCBI Official Synonym Symbols
alpha-kl
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NCBI Protein Information
klotho; secreted form of Klotho protein
UniProt Protein Name
Klotho
Protein Family
UniProt Gene Name
UniProt Entry Name
KLOT_MOUSE
UniProt Comments for Kl
Function: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Ref.1 Ref.5 Ref.6 Ref.9 Ref.14 Ref.15The Klotho peptide generated by cleavage of the membrane-bound isoform maybe an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling. Ref.1 Ref.5 Ref.6 Ref.9 Ref.14 Ref.15

Catalytic activity: A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol. Ref.13 Ref.14

Enzyme regulation: Inhibited by D-saccharic acid 1,4-lactone and taurocholic acid. Ref.13

Subunit structure: Homodimer. Interacts with FGF23 and FGFR1. Ref.12 Ref.15

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Note: Isoform 1 shedding leads to a soluble peptide. Ref.1 Ref.4 Ref.8 Ref.11 Ref.12 Ref.14Isoform 2: Secreted Ref.1 Ref.4 Ref.8 Ref.11 Ref.12 Ref.14. Klotho peptide: Secreted Ref.1 Ref.4 Ref.8 Ref.11 Ref.12 Ref.14.

Tissue specificity: Membrane-bound protein is present in distal renal tubules, inner ear, ependymal cells of brain choroid plexus, elongating spermatids and mature oocytes (at protein level). Soluble peptide is present in serum (100 pM) and cerebrospinal fluid. Expressed strongly in kidney, moderately in brain choroid plexus, and at low levels in pituitary, placenta, skeletal muscle, urinary bladder, aorta, pancreas, testis, ovary, colon, thyroid gland and adipocytes. Ref.1 Ref.2 Ref.4 Ref.7 Ref.8 Ref.11 Ref.12 Ref.14

Developmental stage: Not expressed in the embryo. Expressed in the kidney of newborns. Ref.1

Induction: Induced by 1,25-dihydroxyvitamin D3 in kidney. Down-regulated by angiotensin II and up-regulated by statins through modulation of the RhoA pathway in epithelial cells (in vitro). Isoform 1 (but not isoform 2)is up-regulated by thyroid hormone in adipocytes. Ref.7 Ref.9 Ref.10 Ref.13

Domain: Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 241, and the second one lacks the essential Glu active site residue at position 874.

Post-translational modification: N-glycosylated. Ref.12

Disruption phenotype: Mice display a syndrome resembling to human aging, with short lifespan, infertility, atherosclerosis, skin atrophy, osteoporosis and emphysema. They have various metabolic abnormalities, including increased insulin sensitivity and decreased insulin production. Mice overexpressing Kl have increased resistance to insulin and IGF1, a lifespan extended of more than 20%, and generate fewer offspring. Ref.5

Sequence similarities: Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Biophysicochemical propertiesKinetic parameters:KM=0.249 mM for 4-methylumbelliferylglucuronide Ref.13KM=0.251 mM for estrone 3-beta-D-glucuronideKM=0.174 mM for beta-estradiol 3-beta-D-glucuronideKM=0.251 mM for estriol 3-beta-D-glucuronideVmax=0.62 µM/h/µg enzymepH dependence:Optimum pH is 5.5.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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