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anti-Mouse KLRG1 Monoclonal Antibody

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Catalog # MBS673575
Unit / Price
  0.5 mg  /  $280 +1 FREE 8GB USB
anti-KLRG1 antibody
Product Name

KLRG1, Monoclonal Antibody

Full Product Name

Hamster Anti-Mouse KLRG1/MAFA-UNLB

Product Synonym Names
Hamster Anti-Mouse KLRG1/MAFA - Purified, Unlabeled Antibody; Hamster Anti-Mouse KLRG1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
Syrian Hamster IgG
Clone Number
2F1
Host
Syrian Hamster
Species Reactivity
Mouse
Specificity
KLRG1 (Mr 30-38 kDa)
The monoclonal antibody 2F1 reacts with mouse killer cell lectin-like receptor G1 (KLRG1; formerly known as mouse MAFA or 2F1-Ag), a homodimeric member of the lectin-like type 2 transmembrane receptor family that contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains.1, 2 These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1.3, 4 The 2F1 antibody stains 30-60% of NK1.1+CD3- splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL).1, 5 Cell surface expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC class I expression is indirect, and can be mediated by interactions with class I-specific Ly49 inhibitory receptors.1
Form/Format
Purified (UNLB) Antibody
Immunogen
Activated NK (A-LAK) cells from B6 (H-2^b) mice
Characterization
To ensure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Other Notes
Small volumes of anti-KLRG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-KLRG1 antibody
Flow Cytometry; Immunoprecipitation
Application Notes for anti-KLRG1 antibody
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Product References and Citations for anti-KLRG1 antibody
1. Corral, L., T. Hanke, R.E. Vance, D. Cado, and D.H. Raulet. 2000. Eur. J. Immunol. 30:920.
2. Hanke, T., L. Corral, R.E., Vance, and D.H. Raulet. 1998. 28:4409.
3. Burshtyn, D., A. Scharenberg, N. Wagtmann, S. Rajagopalan, M. Peruzzi, J.-P. Kinet, and E.O. Long. 1997. Immunity 4:77.
4. Nakamura, M.C., E.C. Niemi, M.J. Fisher, L.D. Schultz, W.E. Seaman, and J.C. Ryan. 1997. J. Exp. Med. 185:673.
5. Liu, S., and D.H. Raulet. Personal communication.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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