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anti-Mer antibody :: Rat anti-Mouse Mer Monoclonal Antibody

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Catalog # MBS690563
Unit / Price
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  0.1 mg  /  $490 +1 FREE 8GB USB
Testing Data
Product Name

Mer, Monoclonal Antibody

Popular Item
Full Product Name

Rat Anti-Mouse Mer

Product Synonym Names
Mertk; Eyk; Mer; Nyk; nmf12
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 2; NC_000002.12 (111898479..112039716). Location: 2q14.1
OMIM
604705
3D Structure
ModBase 3D Structure for Q12866
Clonality
Monoclonal
Isotype
IgG1
Clone Number
(3D07)
Host
Rat
Species Reactivity
Mouse
Specificity
This antibody recognizes mouse Mer in western blot. It has no crossreactivity with rmAxl or rmDtk. Cross reactivity to Mer of other species has not been tested!
Purity/Purification
Protein G affinity chromatography
Form/Format
lyophilized
Antibody Generation
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with purified recombinant mouse Mer extracellular domain. IgG1 fraction of the culture supernatant was purified by Protein G affinity chromatography.
Antigen
Recombinant Mouse Mer extracellular domain
Reconstitution
Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.
Buffer
PBS (sterile)
Remarks
This antibody was selected for its ability to detect mouse Mer.
Preparation and Storage
Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity. Avoid repeated freeze and thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-Mer antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Mer antibody
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anti-coagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.
Applications Tested/Suitable for anti-Mer antibody
Western Blot (WB), Immunohistochemistry (IHC) - Paraffin
Application Notes for anti-Mer antibody
1. Western blot: Yes (1:100-1000) 2. Immunohistochemistry (Paraffin): Yes (1:50-200)

Testing Data of anti-Mer antibody
anti-Mer antibody Testing Data image
NCBI/Uniprot data below describe general gene information for Mer. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
110,249 Da
NCBI Official Full Name
tyrosine-protein kinase Mer
NCBI Official Synonym Full Names
c-mer proto-oncogene tyrosine kinase
NCBI Official Symbol
MERTK  [Similar Products]
NCBI Official Synonym Symbols
MER; RP38; c-mer
  [Similar Products]
NCBI Protein Information
tyrosine-protein kinase Mer; STK kinase; proto-oncogene c-Mer; MER receptor tyrosine kinase; receptor tyrosine kinase MerTK
UniProt Protein Name
Tyrosine-protein kinase Mer
UniProt Synonym Protein Names
Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK
Protein Family
UniProt Gene Name
MERTK  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
MERTK_HUMAN
NCBI Summary for Mer
This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP). [provided by RefSeq, Jul 2008]
UniProt Comments for Mer
Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. Ref.11

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Interacts (upon activation) with TNK2; stimulates TNK2 autophosphorylation. Interacts (via N-terminus) with extracellular ligands LGALS3, TUB, TULP1 and GAS6

By similarity. Interacts with VAV1 in a phosphotyrosine-independent manner. Ref.6 Ref.7 Ref.8 Ref.14

Subcellular location: Membrane; Single-pass type I membrane protein

By similarity.

Tissue specificity: Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. Highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver.

Post-translational modification: Autophosphorylated on Tyr-749, Tyr-753 and Tyr-754 in the activation loop allowing full activity. Autophosphorylated on Tyr-872 leading to recruitment of downstream partners of the signaling cascade such as PLCG2

By similarity. Ref.5

Involvement in disease: Retinitis pigmentosa 38 (RP38) [MIM:613862]: A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.3

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.Contains 2 fibronectin type-III domains.Contains 2 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 protein kinase domain.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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