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anti-HLA-DQB1 antibody :: Rat anti-Mouse MHC Class II Monoclonal Antibody

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Catalog # MBS673639
Unit / Price
  0.5 mg  /  $320 +1 FREE 8GB USB
anti-HLA-DQB1 antibody
Product Name

MHC Class II (HLA-DQB1), Monoclonal Antibody

Also Known As

Rat Anti-Mouse MHC Class II-BIOT

Product Synonym Names
Rat Anti-Mouse MHC Class II - Biotin; Rat Anti-Mouse MHC Class II
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
229
Chromosome Location
Chromosome: 6; NC_000006.11 (32627657..32634466, complement). Location: 6p21.3
3D Structure
ModBase 3D Structure for O19707
Clonality
Monoclonal
Isotype
Rat IgG2b
Clone Number
NIMR-4
Host
Rat
Species Reactivity
Mouse
Specificity
MHC Class II molecules
The monoclonal antibody NIMR-4 reacts specifically with a non-polymorphic I-A-encoded epitope on MHC Class II antigens. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells and certain epithelial cells.
Form/Format
Biotin (BIOT) Conjugate
Characterization
To ensure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Other Notes
Small volumes of anti-HLA-DQB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-HLA-DQB1 antibody
Flow Cytometry
Application Notes for anti-HLA-DQB1 antibody
Research Application(s): Complement-dependent cytotoxicity assays

Flow Cytometry Suggested Dilution: <=1 ug/10^6 cells
NCBI/Uniprot data below describe general gene information for HLA-DQB1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
26,489 Da[Similar Products]
NCBI Official Full Name
MHC class II
NCBI Official Synonym Full Names
major histocompatibility complex, class II, DQ beta 1
NCBI Official Symbol
HLA-DQB1  [Similar Products]
NCBI Official Synonym Symbols
IDDM1; CELIAC1; HLA-DQB
  [Similar Products]
NCBI Protein Information
HLA class II histocompatibility antigen, DQ beta 1 chain; MHC DQ beta; MHC class2 antigen; OTTHUMP00000029167; OTTHUMP00000178569; OTTHUMP00000178570; OTTHUMP00000178571; lymphocyte antigen; MHC class II antigen DQB1; MHC class II DQ beta chain; MHC class II antigen HLA-DQ-beta-1; MHC class II HLA-DQ beta glycoprotein; major histocompatibility complex class II beta; HLA class II histocompatibility antigen, DQ beta 1 chain-like
UniProt Protein Name
MHC class II HLA-DQ-beta-1
UniProt Gene Name
HLA-DQB1  [Similar Products]
UniProt Entry Name
O19707_HUMAN
NCBI Summary for HLA-DQB1
HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq]
UniProt Comments for HLA-DQB1
Sequence similarities: Contains 1 Ig-like C1-type (immunoglobulin-like) domain. SAAS SAAS014745
Product References and Citations for anti-HLA-DQB1 antibody
1. R.M.E. Parkhouse. 1996. Personal communication.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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