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anti-PlGF antibody :: Rat anti-Mouse PlGF, blocking antibody Monoclonal Antibody

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Catalog # MBS690032
Unit / Price
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  0.2 mg  /  $705 +1 FREE 8GB USB
Testing Data
Product Name

PlGF, blocking antibody, Monoclonal Antibody

Popular Item
Full Product Name

Rat Anti-Mouse PlGF, blocking antibody

Product Synonym Names
Blocking antibody of mouse PlGF
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 12; NC_000078.6 (85166637..85177785, complement). Location: 12 D; 12 39.58 cM
3D Structure
ModBase 3D Structure for P49764
Clonality
Monoclonal
Isotype
IgG2
Clone Number
(9G33)
Host
Rat
Species Reactivity
Mouse
Specificity
This antibody recognizes mouse PlGF2 in Western blot. No cross reactivity to other species have not been tested!
Purity/Purification
Protein G chromatography
Form/Format
Lyophilized
Reconstitution
Reconstitute the antibody with 400 ul sterile PBS and the final concentration is 500 ug/ml.
Preparation and Storage
Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-PlGF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PlGF antibody
Placenta growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors that share a conserved pattern of eight cysteines. Alternate splicing results in at least three human mature PlGF forms containing 131 (PlGF-1), 152 (PlGF-2), and 203 (PlGF-3) amino acids (aa) respectively. Only PlGF-2 contains a highly basic heparin-binding 21 aa insert at the C-terminus. In the mouse, only one P lGF that is the equivalent of human PlGF-2 has been identified. Mouse PlGF shares 60%, 92%, 62% and 59% aa identity with the appropriate isoform of human, rat, canine and equine PlGF. PlGF is mainly found as variably glycosylated, secreted, 55 - 60 kDa disulfide linked homodimers. Mammalian cells expressing PlGF include villous trophoblasts, decidual cells, erythroblasts, keratinocytes and some endothelial cells. Circulating PlGF increases during human pregnancy, reaching a peak in mid-gestation; this increase is attenuated in preeclampsia. However, deletion of PlGF in the mouse does not affect development or reproduction. Postnatally, mice lacking PlGF show impaired angiogenesis in response to ischemia. PlGF binds and signals through VEGF R1/Flt-1, but not VEGF R2/Flk-1/KDR, while VEGF binds both but signals only through the angiogenic receptor, VEGF R2. PlGF and VEGF therefore compete for binding to VEGF R1, allowing high PlGF to discourage VEGF/VEGF R1 binding and promote VEGF/VEGF R2-mediated angiogenesis. However, PlGF (especially human PlGF-1) and some forms of VEGF can form dimers that decrease the angiogenic effect of VEGF on VEGF R2. PlGF-2, like VEGF164/165, shows heparin-dependent binding of neuropilin (Npn)-1 and Npn-2 and can inhibit nerve growth cone collapse. PlGF induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing, but also contribute to inflammation in active sickle cell disease and atherosclerosis. Circulating PlGF often correlates with tumor stage and aggressiveness, and therapeutic PlGF antibodies are being investigated to inhibit tumor growth and angiogenesis. The functional antibody will block the binding of PlGF to Flt-1/VEGFR-1
Application Notes for anti-PlGF antibody
1. Blocking PlGF-2 induced VEGFR-1 phosphorylation in endothelial cells 2. Western blot: Yes Optimal dilutions should be determined by each laboratory for each application.

Testing Data of anti-PlGF antibody
anti-PlGF antibody Testing Data image
NCBI/Uniprot data below describe general gene information for PlGF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
17,876 Da
NCBI Official Full Name
placenta growth factor isoform 1
NCBI Official Synonym Full Names
placental growth factor
NCBI Official Symbol
NCBI Official Synonym Symbols
PIGF; Plgf; AI854365
  [Similar Products]
NCBI Protein Information
placenta growth factor
UniProt Protein Name
Placenta growth factor
UniProt Gene Name
UniProt Synonym Gene Names
Plgf; PlGF  [Similar Products]
UniProt Entry Name
PLGF_MOUSE
UniProt Comments for PlGF
Function: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth

By similarity.

Subunit structure: Antiparallel homodimer; disulfide-linked. Also found as heterodimer with VEGFA/VEGF

By similarity.

Subcellular location: Secreted

By similarity.

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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